Skip to main content
SpringerLink
Log in

Comparison of ethylene-producing Pseudomonas syringae strains isolated from kudzu (Pueraria lobata) with Pseudomonas syringae pv. phaseolicola and Pseudomonas syringae pv. glycinea

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

The relationships among strains of Pseudomonas syringae pv. glycinea (Psg) and Pseudomonas syringae pv. phaseolicola (Psp) isolated from kudzu ( Pueraria lobata) and bean ( Phaseolus vulgaris) were investigated. All strains tested showed a close phenotypic similarity, with the exception of the utilization of inositol and mannitol as well as the production of toxins. On this basis the strains could be divided into three groups. Group 1 consists of all strains of pathovar glycinea, group 2 includes all Psp strains isolated from kudzu, and all Psp strains isolated from bean belong to group 3. This grouping was also reflected in the genetic fingerprints using the polymerase chain reaction (PCR) with primers that anneal to dispersed repetitive bacterial sequences (rep-PCR). The rep-PCR generated fingerprints were unique for each of the three groups. The strains of group 2, Psp strains isolated from kudzu, possess certain characteristics of group 1 (ethylene production) and group 2 (phaseolotoxin production). The Psp strains from kudzu can be clearly differentiated from Psp strains isolated from bean. They utilize mannitol, produce ethylene, and are strongly pathogenic to kudzu, bean, and soybean. The results obtained show that the Psp strains from kudzu should be separated from the pathovar phaseolicola and should represent their own pathovar.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arndt H, Henning C, Völksch B and FritscheW(1989) Beziehungen zwischen Virulenz und Phaseolotoxinbildungsvermögen bei verschiedenen Pseudomonas syringae pv. phaseolicola-Stämmen. Arch. Phytopathol. Pflanzenschutz 25: 347-357

    Google Scholar 

  • Bradbury JF (1988) Identification of cultivable bacteria from plants and plant tissue cultures by use of simple classical methods. Acta Horticulturae 225: 27-37

    Google Scholar 

  • Dye DW (1968) A taxonomic study of the genus Erwinia. 1. The 'amylovora' group. N.Z.J. Science 11: 590-607

    Google Scholar 

  • Gardan L, Shafik HL and Grimont PAD (1995) DNA relatedness among pathovars of P. syringae and related bacteria. 5th Int. Conf. on Pseudomonas syringae pathovars and related pathogens. Berlin, Germany, Book of abstracts, 42

  • Goto M and Hyodo H(1987) Ethylene production by cell-free extract of the kudzu strain of Pseudomonas syringae pv. phaseolicola. Plant Cell Physiol 28: 405-414

    Google Scholar 

  • Guthke R, Nüske J, Schorcht R, Fritsche W and Knorre WA (1984) Dynamic model of discontinuous and continuous phaseolotoxin production of Pseudomonas syringae pv. phaseolicola. Z Allg Mikrobiol 24: 427-435

    Google Scholar 

  • King EO, Ward MK and Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clinical Medicine 44: 301-307

    Google Scholar 

  • Lelliott RA, Billing E and Hayward AC (1966) A determinative scheme for the fluorescent plant pathogenic Pseudomonads. J Appl Bacteriol 29: 470-489

    Google Scholar 

  • Louws FJ, Fulbright DW, Stephens CT and de Bruijn FJ (1994) Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Appl Environ Microbiol 60: 2286-2295

    Google Scholar 

  • Patil SS, Kolattukudy PE and Dimond AE (1970) Inhibition of ornithine carbamyl transferase from bean plants by the toxin of Pseudomonas phaseolicola. Plant Physiol 46: 752-753

    Google Scholar 

  • Takikawa Y, Ando Y, Hamaya E, Tsuyumu S and Goto M (1988) Identification of the pathogens responsible for bacteriosis of tea plant occurred in 1983. Ann Phytopath Soc Japan 54: 223-228

    Google Scholar 

  • Umbarger HE (1978) Amino acid biosynthesis and its regulation. Annu Rev Biochem 47: 533-606

    Google Scholar 

  • Versalovic J, Koeuth T and Lupski JR (1991) Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19: 6823-6831

    Google Scholar 

  • Völksch B, Bublitz F and Fritsche W (1989) Coronatine production by Pseudomonas syringae pathovars: Screening method and capacity of product formation. J Basic Microbiol 29: 463-468

    Google Scholar 

  • Völksch B, Laplace F and Fritsche W (1984) Untersuchungen zur Variabilität der Phaseolotoxinbildung bei Pseudomonas syringae pv. phaseolicola. Zbl Mikrobiol 139: 109-118

    Google Scholar 

  • Weingart H and Völksch B (1997) Ethylene production by Pseudomonas syringae pathovars in vitro and in planta. Appl Environ Microbiol 63: 156-161

    Google Scholar 

  • Young JM, Bradbury JF, Davis RE, Dickey RS, Ercolani GL, Hayward AC and Vidaver AK (1991) Nomenclatural revisions of plant pathogenic bacteria and list of names 1980-1988. Rev Plant Pathol 70: 211-221

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Mikrobiologie, Friedrich-Schiller-Universität Jena, Philosophenweg 12, D-07743, Jena, Germany

    B. Völksch & H. Weingart

Authors
  1. B. Völksch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Weingart
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Völksch, B., Weingart, H. Comparison of ethylene-producing Pseudomonas syringae strains isolated from kudzu (Pueraria lobata) with Pseudomonas syringae pv. phaseolicola and Pseudomonas syringae pv. glycinea. European Journal of Plant Pathology 103, 795–802 (1997). https://doi.org/10.1023/A:1008686609138

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008686609138

Search

Navigation