Abstract
The genetic origin of cultivated papaya is not clear. Wild relatives of papaya (Carica papaya) from central southern America were investigated using isozyme and RAPD analysis. Seven other species (including six from the genus Carica) were found to be relatively distant from papaya providing no indication of the genetic origin of papaya. Isozyme and RAPD data gave similar measures of genetic similarity within this group. C. papaya was about 70% dissimilar to the other Carica species by both methods. The other Carica species had average dissimilarities around 50%. Two species, C. pubescens and C. stipulata were much closer to each other with similarities of 87% by isozyme analysis and 82% by RAPD analysis. Although both methods gave similar measures for genetic distance the large number of RAPD markers available made RAPD analysis more reliable for analysis of the extremes (e.g. closely related taxa may show no isozyme differences and distant taxa may show no isozyme similarities).
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References
Abo-elwafa, A., K. Murai & T. Shimada, 1995. Intra-and interspecific variations in Lens revealed by RAPD markers. Theor. Appl. Genet. 90: 335–340.
Badilo, V., 1971. Monographia de la familia Caricaceae. Maracay, Venezuela.
Drew, R.A., 1992. Improved techniques for in vitro propagation and germplasm storage of papaya. HortScience 27: 1122–1124.
Graham, G.C., P. Mayers & R.J. Henry, 1994. A simplified method for the preparation of fungal genomic DNA for PCR and RAPD analysis. Biotechniques 16: 48–50.
Hebert, P.D.N. & M.J. Beaton, 1989. Methodologies for allozyme analysis using cellulose acetate electrophoresis. Apractical handbook. Helena Laboratories, Beaumont, Texas.
Henry, R.J., 1996. Practical Applications of Plant Molecular Biology, Chapman and Hall, London.
Horovitz, S. & H. Jimenez, 1967. EsCruzamientos interspecificos e intergen.ricos en Caricaceas y sus implicaciones fitot.cnicas. Agronomia Tropical (Maracay) 17: 323–343.
Jordan, M., 1992. Micropropagation of Papaya (Carica spp) In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry 18-High Tech and Micropropagation II, pp. 441–459, Springer-Verlag, New York.
Khuspe, S.S., R.R. Hendre, A.F. Mascarenhas, V. Jagannathan, M.V. Thombre & A.B. Joshi, 1980. Utilization of tissue culture to isolate interspecific hybrids in Carica L. In: P.S. Rao, M.R. Heble & M.S. Chadla (Eds.), Plant Tissue Culture, Genetic Manipulation and Somatic Hybridisation of Plant Cells, pp. 198–205, Bhamba Atomic Research Centre: India.
Manshardt, R.M. T.F. & Wenslaff, 1989. Zygotic polyembryony in interspecific hybrids of Carica papaya and C. cauliflora. J.Amer. Soc. Hort. Sci. 114: 684–689.
Manshardt, R.M., 1992. Papaya In: F.A. Hammerschlag & R.E. Litz (Eds.), Biotechnology of perennial Fruit Crops, pp. 489–511. Cambridge University Press: Oxford.
Peakall, R., P.E. Smouse & D.R. Huffs, 1995. Evolutionary implications of allozyme and RAPD variation in diploid populations of dioecious buffalograss Buchloe dactyloides. Molecular Ecology 4: 135–147.
Richardson, B.J., P.R. Baverstock & M. Adams, 1986. Allozyme electrophoresis. A handbook for animal systematics and population studies. Academic Press, New York.
Russell, J.R., F. Hosein, E. Johnson, R. Waugh & W. Powell, 1993. Genetic differentiation of cocoa (Theobroma cacao L.) populations revealed by RAPD analysis. Molecular Ecology 2: 89–97.
Saitou, N. & M. Nei, 1987. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406–425.
Shaw, C.R. & R. Prasad, 1970. Starch gel electrophoresis of enzymes. Acompilation of recipes. Biochemical Genetics 4: 297–320.
Sokal, R.R. & P.H.A. Sneath, 1963. Principles of Numeric Taxonomy. Freeman, San Francisco. 359 pp.
Soltis, D.E., C.H. Haufler, D.C. Darrow & G.J. Gastony, 1983. Starch gel electrophoresis of ferns: A compilation of grinding buffers, gel and electrode buffers, and staining schedules. American Fern Journal 73: 9–27.
Sondur, S.N., R.M. Manshardt & J.I. Stiles, 1995. Genetics of growth rate and flowering time in papaya (Carica papaya L.). Journal of Quantitative Trait Loci 1: 4.
Sneath, P.H.A. & R.R. Sokal, 1973. Numerical Taxonomy. Freeman, San Francisco. 573 pp.
Stiles, J.I., C. Lemme, S. Sondur, M.B. Morshidi & R. Manshardt, 1993. Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars. Theor. Appl. Genet. 85: 697–701.
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531–6535.
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Jobin-Decor, M., Graham, G., Henry, R. et al. RAPD and isozyme analysis of genetic relationships between Carica papaya and wild relatives. Genetic Resources and Crop Evolution 44, 471–477 (1997). https://doi.org/10.1023/A:1008644901727
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DOI: https://doi.org/10.1023/A:1008644901727