Abstract
Nineteen natural populations of Fagopyrum homotropicum, a self-fertilizing close relative of common buckwheat, from the Yunnan and Sichuan provinces of China were investigated for their chromosome number and allozyme variation at 16 loci of 11 enzymes. Three populations, 'Deqin', 'Zhongdian', and 'Xiancheng', were revealed to be allotetraploid. Judging from allozyme constitution of the tetraploid and their possible progenitors, diploid progenitors are probably the diploid population of F. homotropicum from Lijiang and a natural population of F. esculentum ssp. ancestralis. Diploid populations of F. homotropicum are fixed for a given allele at almost all isozyme loci. Allozyme variation has been maintained in natural populations mainly by fixing different alleles in different populations, as they are highly differentiated among the populations (Gst = 0.969). The position of populations in the phylogenetic tree constructed from genetic distance nearly corresponded with the geographical position of the populations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bennett, S.T., A.Y. Kenton & M.D. Bennett, 1992. Genomic in situ hybridization reveals the alloploid nature of Milium montianum (Gramineae). Chromosoma 101: 420–424.
Campbell, C., 1995. Inter-specific hybridization in the genus Fagopyrum. Proc. 6th Intl. Symp. on Buckwheat at Ina, pp. 255–263.
Felsenstein, J., 1993. PHYLIP (Phylogeny Inference Package) ver. 3.5c. Distributed by the author, Dept. Genet., Univ. of Washington, Seattle, WA.
Gottlieb, L.D., 1973. Genetic differentiation, sympatric speciation, and the origin of a diploid species of Stephanomeria. Am. J.Bot. 60: 545–553.
Gottlieb, L.D., 1981. Electrophoretic evidence and plant populations. Progr. Phytochem. 7: 1–46.
Hamrick, J.L. & J.W. Godt, 1989. Allozyme diversity in plant species. In: Brown, A.H.D., M.T. Clegg, A.L. Kehler & B.S. Weir (Eds.) Plant Population Genetics, Breeding, and Genetic Resources, Sinauer Assoc. Inc., MA, pp 43–63.
Hirose, T., B.S. Lee, I. Okuno, A. Konishi, M. Minami & A. Ujihara, 1995. Interspecific pollen-pistil interaction and hybridization in genus Fagopyrum.Proc. 6th Intl. Symp. on Buckwheat at Ina, pp. 239–245.
Kamisugi, Y. & K. Fukui, 1990. Automatic karyotyping of plant chromosomes by image techniques. Biol. Techniques 8: 290–295.
Loveless, M.D. & J.L. Hamrick, 1984. Ecological determinants of genetic structure in plant populations. Ann. Rev. Ecol. Syst. 15: 65–95.
Nei, M., 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70: 3321–3323.
Nei, M., 1987. Molecular Evolutionary Genetics. Columbia Univ. Press, New York, NY.
Ohnishi, O., 1988. Population genetics of cultivated common buckwheat, Fagopyrum esculentum Moench. VII. Allozyme variability in Japan, Korea and China. Jpn. J. Genet. 63: 507–522.
Ohnishi, O., 1989. Cultivated buckwheat species and their relatives in the Himalaya and southern China. Proc. 4th Intl. Symp. on Buckwheat at Orel, pp. 562–571.
Ohnishi, O., 1995. Discovery of new Fagopyrum species and its implication for the studies of evolution of Fagopyrum and of the origin of cultivated buckwheat. Proc. 6th Intl. Symp. on Buckwheat at Ina, pp. 175–190.
Ohnishi, O., 1998a. Search for the wild ancestor of buckwheat. I. Description of new Fagopyrum species and their distribution in China. Fagopyrum 15: 18–28.
Ohnishi, O., 1998b. Search for the wild ancestor of buckwheat. III. The wild ancestor of cultivated common buckwheat, and of tartary buckwheat. Econ. Bot. 52: 123–133.
Ohnishi, O. & Y. Matsuoka, 1996. Search for the wild ancestor of buckwheat. II. Taxonomy of Fagopyrum (Polygonaceae) species based on morphology, isozymes and cpDNA variability. Genes & Genetic Systems 71: 383–390.
Ohnishi, O. & T. Nishimoto, 1988. Population genetics of cultivated common buckwheat, Fagopyrum esculentum Moench. V. Further studies on allozyme variability in the Indian and Nepali Himalayas.Jpn. J. Genet. 63: 51–66.
Saito, N. & M. Nei, 1987. The neighbour joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406–425.
Schwarzacher, T., A.R. Leitch, M.D. Bennett & J.S. Heslop-Harrison, 1989. In situ hybridization of parental genomes in a wide hybrid. Ann. Bot. 64: 315–324.
Sinkovic, T. & B. Bohanec, 1988. Chromosome counts and karyotype analysis in buckwheat (Fagopyrum esculentum Moench). Fagopyrum 8: 20–22.
Soltis, D.E. & L.H. Rieseberg, 1986. Autopolyploidy in Tolmiea menziesii (Saxifragaceae): genetic insights from enzyme electrophoresis. Am. J. Bot. 73: 310–318.
Woo, S.H., Q.S. Tsai & T. Adachi, 1995. Possibility of interspecific hybridization by embryo rescue in genus Fagopyrum. Proc. 6th Intl. Symp. on Buckwheat at Ina, pp. 225–237.
Yasui, Y. & O. Ohnishi, 1998. Interspecific relationships in Fagopyrum (Polygonaceae) revealed by the nucleotide sequences of rbcL and accD genes and their intergenic region. Am. J. Bot. 85: 1134–1142.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ohnishi, O., Asano, N. Genetic diversity of Fagopyrum homotropicum, a wild species related to common buckwheat. Genetic Resources and Crop Evolution 46, 389–398 (1999). https://doi.org/10.1023/A:1008640522979
Issue Date:
DOI: https://doi.org/10.1023/A:1008640522979