An understanding of the genetic structure of populations is vital for the formation of optimum collection, conservation and utilization strategies for plant genetic resources. This is of particular importance in the case of in-situ conservation, a strategy gaining in popularity. The population genetic structures of five wild lentil taxa, Lens culinaris subsp. orientalis, L. odemensis, L. ervoides, L. nigricans and L. lamottei were investigated using isozyme electrophoresis and random amplified polymorphic DNA (RAPD). Approximately 20 plants from each of 5 populations per taxon were screened for variation at 11 isozyme loci and using three RAPD primers. Levels of variation were generally low, although considerable variation existed in the levels of diversity found within populations of L. culinaris subsp. orientalis and L. lamottei. Comparison of the results obtained in this study with the results obtained in a previous study indicate that this is a trend occurring across all species. It implies that levels of diversity within populations must be measured and considered prior to targeting of specific populations for in-situ conservation. Analysis of molecular variance of both isozyme and RAPD data revealed that between 78% and 99% of the variation was attributable to between-population differences. Isozyme results from L. lamottei populations were, however, contradictory. Possible explanations for this difference are discussed.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Abo-elwafa, A., K. Murai & T. Shimada, 1995. Intra-and interspecific variations in Lens revealed by RAPD markers. Theor. Appl. Genet. 90: 335-340.
Affre, L. & J.D. Thompson, 1997. Variation in the population genetic structure of two Cyclamen species on the island of Corsica. Heredity 78: 205-214.
Ahmad, M., D.L. McNeil, A.G. Fautrier, K.F. Armstrong & A.M. Paterson, 1996. Genetic relationships in Lens species and parentage determination of their interspecific hybrids using RAPD markers. Theor. Appl. Genet. 92: 1091-1098.
Chalmers, K.J., R. Waugh, J.I. Sprent, A.J. Simons & W. Powell, 1992. Detection of genetic variation between and within populations of Gliricidia sepium and G. maculata using RAPD markers. Heredity 69: 465-472.
Chase, C.D., V.M. Ortega & C.E. Vallejos, 1991. DNA restriction fragment length polymorphism correlate with isozyme diversity in Phaseolus vulgaris L. Theor. Appl. Genet. 81: 806-811.
Clegg, M.T., 1989. Molecular diversity in plant populations. In: Brown, A.D.H., M. Clegg, A. Kahler & B. Weir (Eds), Plant population genetics, breeding and genetic resources, pp. 98-115, Sinauer Associates, Sunderland, Massachusetts.
Czefranova, Z., 1971. Review of species in the genus Lens Mill. (in Russian). Nov. Sistemat Vysshikh Rastenii 8: 184-191.
Dawson, I.K., K.J. Chalmers, R. Waugh & W. Powell, 1993. Detection and analysis of genetic variation in Hordeum spontaneum populations from Israel using RAPD markers. Mol Ecol. 2: 151-159.
Ertug Firat, A. & A. Tan, 1997. In situ conservation of genetic diversity in Turkey. In: Maxted, N., B.V. Ford-Lloyd & J.G. Hawkes (Eds), Plant genetic conservation, pp. 254-262, Chapman & Hall, London.
Excoffier, L., P.E. Smouse & J.M. Quattro, 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479-491.
Ferguson, M.E., 1998. Studies of genetic variation within the genus Lens. PhD Thesis. School of Biological Sciences, University of Birmingham, Birmingham, UK.
Ferguson, M.E. & L.D. Robertson, 1996. Genetic diversity and taxonomic relationships within the genus Lens as revealed by allozyme polymorphism. Euphytica 91: 163-172.
Ferguson, M., N. Acikgoz, A. Ismail & A. Cinsoy, 1996. An ecogeographic survey of wild Lens species in Aegean and South West Turkey. Anadolu 6: 159-166.
Ferguson, M., B.V. Ford-Lloyd, L.D. Robertson, N. Maxted & H.J. Newbury, 1998a. Mapping of geographical distribution of genetic variation in the genus Lens for the enhanced conservation of plant genetic diversity. Mol. Ecol. (In press).
Ferguson, M.E., L.D. Robertson, B.V. Ford-Lloyd, H.J. Newbury & N. Maxted, 1998b. Contrasting genetic variation amongst lentil landraces from different geographical origins. Euphytica 102: 265-273.
Fofana, B., X. Vekemans, P. du Jardin & J.P. Baudoin, 1997. Genetic diversity in Lima bean (Phaseolus lunatus L.) as revealed by RAPD markers. Euphytica 95: 157-165.
Frankel, O.H., A.H.D. Brown & J.J. Burdon, 1995. The conservation of plant biodiversity. Cambridge University Press, Cambridge, UK.
Furnier, G.R., P. Knowles, M.A. Clyde & B.P. Dancik, 1987. Effects of avian seed dispersal on the genetic structure of whitebark pine populations. Evolution 4: 607-612.
Garcia, P., F.J. Vences, M. Peres dell las Vega & R.W. Allard, 1989. Allelic and genotypic composition of ancestral Spanish and colonial California gene pools of Avena barbata: evolutionary implications. Genetics 122: 687-694.
Hamrick, J.L. & M.J.W. Godt, 1990. Allozyme diversity in plant species. In: Brown, A.H.D., M.T. Clegg, A.L. Kahler & B.S. Weir (Eds), Plant population genetics, breeding and genetic resources, pp. 43-63, Sinauer Associates Inc., Massachusetts.
Hamrick, J.L., Y.B. Linhart & J.B. Mitton, 1979. Relationships between life history characteristics and electrophoreticallydetectable genetic variation in plants. Ann. Rev. Ecol. Sys. 10: 173-200.
Havey, M. & F. Muehlbauer, 1989. Linkages between restriction fragment length, isozyme and morphological markers in lentil. Theor. Appl. Genet. 77: 395-401.
Hoffman, D., D. Soltis, F. Muehlbauer & G. Ladizinsky, 1986. Isozyme polymorphism in Lens (Leguminosae). Syst. Bot. 11: 392-402.
Huff, D.R., R. Peakell & P.E. Smouse, 1993. RAPD variation within and among natural populations of outcrossing buffalograss [Buchloe dactyloides (Nutt.) Englem.]. Theor. Appl. Genet. 86: 927-934.
Jana, S., 1993. Utilization of biodiversity from in-situ reserves, with special reference to wild wheat and barley. In: Damania, A.B (Eds), Biodiversity and wheat improvement, pp. 311-323, John Wiley, Chichester.
Ladizinsky, G., 1993. Wild lentils. Critical Reviews in Plant Sciences 12: 169-184.
Ladizinsky, G., 1997. A new species of Lens from south-east Turkey. Bot. J. Lin. Soc. 123: 257-260.
Ladizinsky, G., D. Braun & F.J. Muehlbauer, 1983. Evidence for the domestication of Lens nigricans (M.Bieb.) Godron in S. Europe. Bot. J. Linn. Soc. 87: 169-176.
Legionnet, A. & F. Lefevre, 1996. Genetic variation of the riparian pioneer tree species Populus nigra L. 1. Study of population structure based on isozymes. Heredity 77: 629-637.
Liu, Z. & G.R. Furnier, 1993. Comparison of allozyme, RFLP, and RAPD markers for revealing genetic variation within and between trembling aspen and bigtooth aspen. Theor. Appl. Genet. 87: 97-105.
Loveless, M.D. & J.L. Hamrick, 1984. Ecological determinants of genetic structure in plant populations. Annu. Rev. Ecol. Sys. 15: 65-95.
Lynch, M. & B.G. Milligan, 1994. Analysis of population genetic structure with RAPD markers. Mol. Ecol. 3: 91-99.
Maxted, N., B. Ford-Lloyd & J.G. Hawkes, 1996. Complementary conservation strategies. In: Maxted, N., B.V. Ford-Lloyd & J.G. Hawkes (Eds), Plant genetic conservation, pp. 15-39, Chapman & Hall, London.
Messmer, M.M., A. E. Melchieger, M. Lee, W.L. Woodman, E.A. Lee & K.R. Lamkey, 1991. Genetic diversity among progenitors and elite lines from the Iowa Stiff Stalk Synthetic (BSSS) maize populations: comparison of allozyme and RFLP data. Theor. Appl. Genet. 83: 97-107.
Michalakis, Y. & L. Excoffier, 1996. A generic estimation of population subdivision using distances between alleles with special reference to microsatellite loci. Genetics 142: 1061-1064.
Mitton, J.B., K.B. Sturgeon & M.L. Davis, 1980. Genetic differentiation in ponderosa pine along a steep elevation transect. Silvae Genet. 29: 3-4.
Muehlbauer, F.J., N.F. Weeden & D. Hoffman, 1989. Inheritance and linkage relationships of several isozyme loci in lentil (Lens Miller). J. Hered. 80: 298-303.
Nei, M., 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci., USA 70: 3321-3323.
Nei, M. & W. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci, USA 76: 5269-5273.
Nesbitt, K.A., B.M. Potts, R.E. Vaillancourt, A.K. West & J.B. Reid, 1995. Partitioning and distribution of RAPD variation in a forest tree species, Eucalyptus globulus (Myrtaceae). Heredity 74: 628-637.
Nevo, E., T. Krugman & A. Beiles, 1994. Edaphic natural selection of allozyme polymorphisms in Aegilops peregrina at a Galilee microsite in Israel. Heredity 72: 109-112.
Pinkas, R., D. Zamir & G. Ladizinsky, 1985. Allozyme divergence and evolution in the genus Lens. Pl. Syst. Evol. 151: 131-150.
Raybould, A.F., R.J. Mogg & R.T. Clarke, 1996. The genetic structure of Beta vulgaris ssp. maritima (sea beet) populations: RFLPs and isozymes show different patterns of gene flow. Heredity 77: 245-250.
Schneider, S., J. Kueffer, D. Roessli & L. Excoffier, 1997. Arlequin. University of Geneva, Geneva (http://anthropologie.unige.ch/arlequin).
Schoen, D.J. & A.H.D. Brown, 1993. Conservation of allelic richness in wild crop relatives is aided by assessment of genetic markers. Proc. Natl. Acad. Sci., USA 90: 10623-10627.
Sharma, S.K., I.K. Dawson & R. Waugh, 1995. Relationships among cultivated and wild lentils revealed by RAPD analysis. Theor. Appl. Genet. 91: 647-654.
Soltis, P.S. & D.E. Soltis, 1988. Genetic variation and population structure in the fern Blechnum spicant (Blechnaceae) from Western North America. Amer. J. Bot. 75: 37-44.
Tadmor, Y., D. Zamir & G. Ladizinsky, 1987. Genetic mapping of an ancient translocation in the genus Lens. Theor. Appl. Genet. 73: 883-892.
Tahir, M. & F.J. Muehlbauer, 1994. Gene mapping in lentil with recombinant inbred lines. J. Hered. 85: 306-310.
UNCED, 1992. Convention on Biological Diversity. United Nations Conference on Environment and Development, Geneva.
Vaillancourt, R.E. & A.E. Slinkard, 1992. Inheritance of new genetic markers in lentil (Lens Miller). Euphytica 64: 227-236.
van Dijk, H., K. Wolff & A. de Vries, 1988. Genetic variability in Plantago species in relation to their ecology. 3. Genetic structure of populations of P. major, P. lanceolata and P. coronopus. Theor. Appl. Genet. 75: 518-528.
Van Oss, H., Y. Aron & G. Ladizinsky, 1997. Chloroplast DNA variation and evolution in the genus Lens Mill. Theor. Appl. Genet. 94: 452-457.
Weir, B.S. & C.C. Cockerham, 1984. Estimating F-statistics for the analysis of population structure. Evolution 38: 1358-1370.
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuc. Acids Res. 18: 6531-6535.
Wilson, V.E. & A.G. Law, 1972. Natural crossing in Lens esculenta Moench. J. Amer. Soc. Hort. Sci. 97: 142-143.
Zamir, D. & G. Ladizinsky, 1984. Genetics of allozyme variants and linkage groups in lentil. Euphytica 33: 329-336.
About this article
Cite this article
Ferguson, M.E., Newbury, H.J., Maxted, N. et al. Population genetic structure in Lens taxa revealed by isozyme and RAPD analysis. Genetic Resources and Crop Evolution 45, 549–559 (1998). https://doi.org/10.1023/A:1008640201896
- population genetic structure