Skip to main content
SpringerLink
Log in

High level of resistance in potato to potato mop-top virus induced by transformation with the coat protein gene

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

Transgenic plants of potato cvs Saturna and Pentland Marble expressing the potato mop-top virus (PMTV) coat protein (CP) gene were produced. Plants contained transgene RNA transcript and CP. In resistance tests made in a screenhouse, plants were grown in pots containing field soil infested with viruliferous Spongospora subterranea (the vector of PMTV). PMTV infection of the daughter tubers was determined by ELISA; 10% and 17%, respectively, of tubers from non-transformed control plants of Saturna and Pentland Marble became infected. However, tubers from six transgenic Saturna lines were immune to PMTV infection, and only one transgenic Pentland Marble tuber of 261 tested from four lines was infected. Spraing symptoms were not seen in any Saturna tubers, but less than 1% of transgenic Pentland Marble tubers developed symptoms compared to 12% of those from control Pentland Marble plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Acosta O, Barker H and Mayo MA (1995) Prospects for improving virus resistance of potato crops in Colombia by transgenic technology. Fitopatologia Colombiana 18: 66-77

    Google Scholar 

  • Arif M, Torrance L and Reavy B (1995) Acquisition and transmission of potato mop-top furovirus by a culture of Spongospora subterraneaf. sp. subterraneaderived from single cystosorus. Ann Appl Biol 126: 493-503

    Google Scholar 

  • Arli M (1996) Studies on the detection and replication of potato mop-top virus. PhD thesis, University of Dundee, UK.

  • Barker H, Reavy B, Kumar A, Webster KD and Mayo MA (1992) Restricted virus multiplication in potatoes transformed with the coat protein gene of potato leafroll luteovirus: similarities with a type of host gene-mediated resistance. Ann Appl Biol 120: 55- 64

    Google Scholar 

  • Barker H, Reavy B, McGeachy KD and Dawson S (1998) Transformation of Nicotiana benthamianawith the potato mop-top virus coat protein gene produces a novel resistance phenotype mediated by the coat protein. Mol Plant-Microbe Interact 11: 626-633

    Google Scholar 

  • Harrison BD and Jones RAC (1970) Host range and some properties of potato mop-top virus. Ann Appl Biol 65: 393-402

    Google Scholar 

  • Harrison BD and Jones RAC (1971) Factors affecting the development of spraing in potato tubers infected with potato mop-top virus. Ann Appl Biol 68: 281-289

    Google Scholar 

  • Jones RAC and Harrison BD (1969) The behaviour of potato mop-top virus in soil, and evidence for its transmission by Spongospora subterranea(Wallr.) Lagerh. Ann Appl Biol 63: 1-17

    Google Scholar 

  • Lomonossoff GP (1995) Pathogen-derived resistance to plant viruses. Annu Rev Phytopathol 33: 323-343

    Article  Google Scholar 

  • Mayo MA, Torrance LT, Cowan G, Jolly, CA, Macintosh SM, Orrega R, Barrera C and Salazar LF (1996) Conservation of coat protein sequence among isolates of potato mop-top virus from Scotland and Peru. Arch Virol 141: 1115-1121

    PubMed  Google Scholar 

  • Reavy B, Arif M, Kashiwazaki S, Webster KD and Barker H (1995) Immunity to potato mop-top virus in Nicotiana benthamianaplants expressing the coat protein gene is effective against fungal inoculation of the virus. Mol Plant-Microbe Interact 8: 286-291

    PubMed  Google Scholar 

  • Reavy B, Sandgren M, Barker H, Heino P and Oxelfelt P (1997) A coat protein transgene from a Scottish isolate of potato mop-top virus mediates strong resistance against Scandinavian isolates which have similar coat protein genes. Europ J Plant Pathol 103: 829-834

    Article  Google Scholar 

  • Sandgren M (1995) Potato mop-top virus (PMTV): distribution in Sweden, development of symptoms during storage and cultivar trials in field and glasshouse. Pot Res 38: 387-397

    Google Scholar 

  • Torrance L, Cowan GH and Pereira LG (1993) Monoclonal antibodies specific for potato mop-top virus and some properties of the coat protein. Ann Appl Biol 122: 311-322

    Google Scholar 

  • Webster KD and Barker H (1998) Detection and quantification of transcript RNA in transgenic plants using digoxigenin-labeled cDNA probes. In: Foster GD and Taylor SC (eds) Methods in Molecular BiologyVol. XX: Plant Virology (pp 437-445) Humana Press Inc., Totowa, NJ, USA.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK (Fax

    Hugh Barker, Brian Reavy & Kara D. McGeachy

Authors
  1. Hugh Barker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Brian Reavy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kara D. McGeachy
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Barker, H., Reavy, B. & McGeachy, K.D. High level of resistance in potato to potato mop-top virus induced by transformation with the coat protein gene. European Journal of Plant Pathology 104, 737–740 (1998). https://doi.org/10.1023/A:1008627527260

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008627527260

Search

Navigation