Germination of conidia, uredospores and basidiospores of ten diverse fungi were compared in sterile distilled water, 0.5% sucrose, 0.5% mucin and 0.5% xanthan gum. All three compounds generally increased spore germination on cellophane membranes compared to water, but mucin was the most effective. Mucin stimulated significantly more germination than sucrose for 7 of the 10 fungi, and only Aspergillus niger and Colletotrichum gloeosporioides showed greater spore germination in sucrose than in mucin. Sucrose had no effect on uredospore or basidiospore germination, but mucin stimulated germination of these spores. Mucin was more effective than xanthan gum in increasing spore germination for 9 of the 10 fungi. Animal mucin is chemically similar to the natural spore matrix of some fungi and may provide some of the benefits of the spore matrix without the drawback of possible germination inhibitors. Animal mucin may be a useful substitute for the spore matrix in inoculations of a variety of fungi.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Bartnicki-Garcia S (1984) Spore germination in fungi: Basic concepts. In: Roberts DW and Aist JR (eds). Infection Processes of Fungi. (pp. 111–117) The Rockefeller Foundation, New York.
Beckett A, Tatnell JA and Taylor N (1990) Adhesion and pre-invasion behaviour of urediniospores of Uromyces viciae-fabae during germination on host and synthetic surfaces. Mycol Res 94: 865–875.
Braun EJ and Howard RJ (1994) Adhesion of Cochliobolus heterostrophus conidia germlings to leaves and artificial surfaces. Exp Mycol 18: 211–220.
Cardina J (1986) Enhancement of anthracnose severity on Florida beggarweed (Desmodium tortuosum S. W. (DC)). WSSA Abstracts 26: 51.
Chase TE and Ullrich RC (1985) Fruiting and genetic analysis of Heterobasidion annosum. Mycologia 77: 464–471.
Chaubal R, Wilmot VA and Wynn WK (1991) Visualization, adhesiveness, and cytochemistry of the extracellular matrix produced by urediniospores germ tubes of Puccinia sorghii. Can J Bot 69: 2044–2054.
Dhingra OD and Sinclair JB (1995) Basic Plant Pathology Methods. CRC Lewis Publishers, Boca Raton.
Griffin DH (1994) Fungal Physiology. Wiley-Liss, New York.
Kennedy JF and Bradshaw IJ (1984) Production, properties and applications of xanthan. In: Bushell ME (ed.). Progress in Industrial Microbiology: Modern Applications of Traditional Biotechnologies, Vol 19 (pp. 319–371) Elsevier, Amsterdam.
Leite B and Nicholson RL (1992) Mycosporine-alanine: A self-inhibitor of germination from the conidial mucilage of Colletotrichum graminicola. Exp Mycol 16: 76–86.
Louis I, Chew A and Lim G (1988) Influence of spore density and extracellular conidial matrix on spore germination in Colletotrichum capsici. Trans Br Mycol Soc 91: 694–697.
Louis I and Cooke RC (1985a) Conidial matrix and spore germination in some plant pathogens. Trans Br Mycol Soc 84: 661–667.
Louis I and Cooke RC (1985b) Enzymes in the condial matrix of Colletotrichum gloeosporioides and Mycosphaerella pinodes. Trans Br Mycol Soc 84: 742–745.
Moloshok TD, Leinhos GME, Staples RC and Hoch HC (1993) The autogenic extracellular environment of Uromyces appendiculatus urediospore germlings. Mycologia 85: 392–400.
Moloshok TD, Tehune BT, Lamboy JS and Hoch HC (1994) Fractionation of extracellular matrix components from urediospore germlings of Uromyces. Mycologia 86: 787–794.
Mondal AH and Parbery DG (1992) The spore matrix and germination in Colletotrichum musae. Mycol Res 96: 592–596.
Nicholson RL, Butler LG and Asquith TN (1986) Glycoproteins from Collototrichum graminicola that bind phenols: Implications for survival and virulence of phytopathogenic fungi. Phytopathology 76: 1315–1318.
Nicholson RL and Moraes WBC (1980) Survival of Colletotrichum graminicola: Importance of the spore matrix. Phytopathology 70: 255–261.
Porter FM (1969) Protease, cellulase, and differential localization of endo-and exopolygalacturonase in conidia and conidial matrix ofColletotrichum orbiculare. Phytopathology 59: 1209–1213.
Ramadoss CS, Uhlig J, Carlson DM, Butler LG and Nicholson RL (1985) Composition of the mucilaginous spore matrix of Colletotrichum graminicola, a pathogen of corn, sorghum, and other grasses. J Agric Food Chem 33: 728–732.
Sparace SA, Wymore LA, Menassa R and Watson AK (1991) Effects of the Phomposis convolvulus conidial matrix on conidia germination and the leaf anthracnose disease of filed bindweed (Convolvulus arvensis). Plant Dis 75: 1175–1179.
Toribara NW, Roberton AM, Ho SB, Kuo WL, Gum E, Hicks JW, Byrd JC, Siddiki B, and Kim YS (1993) Human gastric mucin: Identification of a unique species by expression cloning. J Biol Chem 268: 5879–5885.
Wilson HA, Lilly VG and Leach JG (1965) Bacterial polysaccharides. IV. Longevity of Xanthomonas phaseoli and Serratia marcescens in bacterial exudates. Phytopathology 55: 1135–1138.
About this article
Cite this article
Mahuku, G., Goodwin, P. Influence of Sucrose, Mucin and Xanthan Gum on Spore Germination of Ten Different Fungi. European Journal of Plant Pathology 104, 849–852 (1998). https://doi.org/10.1023/A:1008620521230
- spore matrix