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Effect of veratryl alcohol and manganese (IV) oxide on ligninolytic activity in semi solid cultures of Phanerochaete chrysosporium

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Abstract

An inert carrier (nylon sponge), a non-inert carrier (barley straw) and the addition of veratryl alcohol or manganese (IV) oxide to the cultures were used to study the production of ligninolytic enzymes by Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) during semi solid state fermentation conditions. By supplementing the medium with these compounds we could stimulate the ligninolytic system of this fungus. The different carriers employed and the effect of adding veratryl alcohol or manganese (IV) oxide to the cultures were compared in order to determine the best system to produce high activities of ligninolytic enzymes. Lignin peroxidase (LiP) activities higher than 500 U/L and manganese-dependent peroxidase (MnP) activities about 1100 U/L were achieved.

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References

  • Archibald FS (1992) A new assay for lignin-type peroxidases employing the dye Azure B. Appl. Environ. Microbiol. 58(9): 3110- 3116

    Google Scholar 

  • Asther M, Bellon-Fontaine MN, Capdevila C & Corrieu G (1990) A thermodynamic model to predict Phanerochaete chrysosporium INA-12 adhesion to various solid carriers in relation to lignin peroxidase production. Biotechnol. Bioeng. 35: 477- 482

    Google Scholar 

  • Barr DP & Aust SD (1994) Mechanisms white rot fungi use to degrade pollutants. Environ. Sci. Technol. 28(2): 78- 87

    Google Scholar 

  • Blanchette RA (1984) Manganese accumulation in wood decayed by white rot fungi. Phytopathology, 74: 725- 730

    Google Scholar 

  • Buswell JA & Odier E (1987) Lignin biodegradation. CRC Crit. Rev. Biotechnol. 6: 1- 60

    Google Scholar 

  • Buswell JA (1987) Fungal degradation of lignin. In: Arora DK, Bharat Rai, Mukerji KG & Knudsen G (Eds) Handbook of Applied Mycology, Vol. 1, Soil and Plants. Marcel Dekker, NY

    Google Scholar 

  • Dass SB & Reddy CA (1990) Characterization of extracellular peroxidases produced by acetate-buffered cultures of the lignin-degrading basidiomycete Phanerochaete chrysosporium. FEMS Microbiol. Lett. 69: 221- 224

    Google Scholar 

  • Dosoretz CG, Chen HC & Grethlein ME (1990) Effect of oxygenation conditions on submerged cultures of Phanerochaete chrysosporium. Appl. Microb. Biotech. 34: 131- 137

    Google Scholar 

  • Eriksson KE, Patterson B, Volc J & Musilek V (1986) Formation and partial characterization of glucose-2-oxidase, a H2O2producing enzyme in Phanerochaete chrysosporium. Appl. Microbiol. Biotechnol. 23: 257- 262

    Google Scholar 

  • Faison BD & Kirk TK (1985) Factors involved in the regulation of a ligninase activity in Phanerochaete chrysosporium. Appl. Environ. Microbiol. 49: 299- 304

    Google Scholar 

  • Faison BD, Kirk TK & Farrell RL (1986). Role of veratryl alcohol in regulating ligninase activity in Phanerochaete chrysosporium. Appl. Environ. Microbiol. 52: 251- 254

    Google Scholar 

  • Farrell RL, Murtagh KE, Tien M, Mozuch MD & Kirk TK (1989) Physical and enzymatic properties of lignin peroxidases isoenzymes from Phanerochaete chrysosporium. Enz. Microb. Technol. 11: 322- 328

    Google Scholar 

  • Feijoo G (1994) Tesis. Producció n en continuo de enzimas ligninolíticos por Phanerochaete chrysosporium. Universidad de Santiago de Compostela, Santiago de Compostela

    Google Scholar 

  • Ferrer P & Solá C (1992) Lipase production by immobilized Candida rugosa cells. Appl. Microb. Biotech. 37: 737- 741

    Google Scholar 

  • Ghose TK (1987) Measurement of cellulase activities. Pure Appl. Chem. 59: 257- 268

    Google Scholar 

  • Glenn JK, Morgan MA, Mayfield MB, Kuwahara M & Gold MH (1983) An extracellular H2O2-requiring enzyme preparation involved in lignin biodegradation by the white-rot basidiomycete Phanerochaete chrysosporium. Biochem. Biophys. Res. Commun. 114: 1077- 1083

    Google Scholar 

  • Gold MH, Kuwahara M, Chiu AA & Glenn JK (1984) Purification and characterization of an extracellular H2O2-requiring diarylpropane oxygenase from the white rot basidiomycete Phanerochaete chrysosporium. Arch. Biochem. Biophys. 234: 353- 362

    Google Scholar 

  • Gold MH, Wariishi H & Valli K (1989) Extracellular peroxidases involved in lignin degradation by white-rot basidiomycete Phanerochaete chrysosporium. In: Whitaker J & Sonnet PE (Eds) Biocatalysis in Agricultural Biotechnology (pp 127- 140). American Chemical Society, Washington DC

    Google Scholar 

  • Jäger A, Croan C & Kirk TK (1985) Production of ligninases and degradation of lignin in agitated submerged cultures of Phanerochaete chrysosporium. Appl. Environ. Microbiol. 50: 1274- 1278

    Google Scholar 

  • Jeffries TW, Choi S & Kirk TK (1981) Nutritional regulation of lignin degradation by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 42: 290- 296

    Google Scholar 

  • Kern HW (1989) Improvement in the production of extracellular lignin peroxidases by Phanerochaete chrysosporium: Effect of solid manganese-IV oxide. Appl. Microbiol. Biotechnol. 32: 223- 234

    Google Scholar 

  • ____ (1990) Production and stability of lignin peroxidases of Phanerochaete chrysosporium cultivated on glycerol in the presence of solid manganese (IV) oxide. Appl. Microbiol. Biotechnol. 33: 582- 588

    Google Scholar 

  • Kirk TK & Farrell, RL (1987) Enzymatic ‘combustion’: The microbial degradation of lignin. Annu. Rev. Microbiol. 41: 465- 505

    Google Scholar 

  • Kirk TK, Croan S, Tien M, Murtagh KE & Farrell RL (1986) Production of multiple ligninases by Phanerochaete chrysosporium: Effect of selected growth conditions and use of a mutant strain. Enz. Microb. Technol. 8: 27- 32

    Google Scholar 

  • Knapp JS & Howell JA (1985) In: Wiseman A & Howard E (Eds) Topics in Enzymes and Fermentation Biotechnology, Vol. 4 (pp 85- 143)

  • Kuwahara M, Glenn JK, Morgan MA & Gold, MH (1984) Separation and characterization of two extracellular H2O2-dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. FEBS Lett. 169: 247- 250

    Google Scholar 

  • Leisola MSA, Kozulic B, Meussdorffer F & Fiechter A (1987) Homology among multiple extracellular peroxidases from Phanerochaete chrysosporium. J. Biol. Chem. 262: 419- 424

    Google Scholar 

  • Leisola MSA, Thanei-Wyss U & Fiechter A (1985) Strategies for production of high ligninase activities by Phanerochaete chrysosporium. Journal of Biotechnology 3: 97- 107

    Google Scholar 

  • Linko S (1992). Production of lignin peroxidase by immobilized P. chrysosporium. Thesis. University of Helsinki, Helsinki

  • Molony AP, O'Rorke A, Considine PJ & Coughlan MP (1984) Enzymic saccharification of sugar beet pulp. Biotechnol. Bioeng. 26: 714- 718

    Google Scholar 

  • Odier E & Delattre M (1990) Multiple lignin peroxidases of Phanerochaete chrysosporium INA- 12. Enz. Microb. Technol. 12: 447- 452

    Google Scholar 

  • Pandey A (1992) Recent process developments in solid-state fermentation. Process Biochemistry 27: 109- 117

    Google Scholar 

  • Pandey A, Nigam P & Vogel M (1988) Simultaneous saccharification and protein enrichment fermentation of sugar beet pulp. Biotechnol. Letts. 10(1): 67- 72

    Google Scholar 

  • Pè rié FH & Gold MH (1991) Manganese regulation of manganese peroxidase expression and lignin degradation by the white rot fungus Dichomitus squalens. Appl. Environ. Mcrobiol. 57: 2240- 2245

    Google Scholar 

  • Reddy CA & Kelley RL (1986) The central role of hydrogen peroxide in lignin biodegradation by Phanerochaete chrysosporium. In: Barry S, Houghton DR, Llewellyn GC & O'Rear CE, (Eds) Biodeterioration CAB (pp 535- 542). International Mycological Institutes, London

    Google Scholar 

  • Renganathan V, Miki K & Gold MH (1985) Multiple molecular forms of diarylpropane oxygenase, an H2O2-requiring, lignin-degrading enzyme from Phanerochaete chrysosporium. Arch. Biochem. Biophys. 342: 304- 314

    Google Scholar 

  • Sarkanen KV & Ludwig CH (1971) In Lignins: Occurrence, Formation and Structure (pp 1- 18). Wiley-Interscience, NY

    Google Scholar 

  • Tien M & Kirk TK (1983) Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium Burds. Science 221: 661- 663

    Google Scholar 

  • ____ (1984) Lignin-degrading enzyme from Phanerochaete chrysosporium. Purification, characterization and catalytic properties of an unique H2O2-requiring oxygenase. Proc. Natl. Acad. Sci. USA 81: 2280- 2284

    Google Scholar 

  • ____ (1988) Lignin peroxidase of Phanerochaete chrysosporium. Meth. Enzymol. 161: 238- 249

    Google Scholar 

  • Tonon F & Odier E (1988) Influence of veratryl alcohol and hydrogen peroxide on ligninase activity and ligninase production by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 54(2): 466- 472

    Google Scholar 

  • Troller J, Smith JG, Leisola SA, Kallen K, Winterhalter KH & Fiechter A (1988) Crystallization of a lignin peroxidase from the white-rot fungus Phanerochaete chrysosporium. Biotechnology. 6: 571- 573

    Google Scholar 

  • Ulmer DC, Leisola MSA & Fiechter A (1984). Possible induction of the ligninolytic system of P. chrysosporium. J. Biotechnol. 1: 13- 24

    Google Scholar 

  • Vares T (1996) Ligninolytic enzymes and lignin-degrading activity of taxonomically different white-rot fungi. Thesis. University of Helsinki, Helsinki

  • Vares T, Kalsi M & Hatakka A (1995) Lignin peroxidases, manganese peroxidases and other ligninolytic enzymes produced by Phlebia radiata during solid state fermentation of wheat straw. Appl. Environ. Microbiol. 61(10): 3515- 3520

    Google Scholar 

  • Weatherburn MW (1967) Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 28: 971- 974

    Google Scholar 

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Authors
  1. Susana Rodríguez Couto
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  2. Marjaana Rättö
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Rodríguez Couto, S., Rättö, M. Effect of veratryl alcohol and manganese (IV) oxide on ligninolytic activity in semi solid cultures of Phanerochaete chrysosporium. Biodegradation 9, 143–150 (1998). https://doi.org/10.1023/A:1008397913262

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