Abstract
A multiple-quantum 3D HCN-CCH-TOCSY experiment is presented for the assignment of RNA ribose resonances. The experiment makes use of the chemical shift dispersion of N1 of pyrimidine and N9 of purine to distinguish the ribose spin systems. It provides an alternative approach for the assignment of ribose resonances to the currently used COSY- and TOCSY-type experiments in which either 13C or 1H is utilized to distinguish the different spin systems. Compared to the single-quantum version, the sensitivity of the multiple-quantum HCN-CCH-TOCSY experiment is enhanced on average by a factor of 2 for a 23-mer RNA aptamer complexed with neomycin.
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Bax, A., Clore, G.M. and Gronenborn, A.M. (1990a) J. Magn. Reson., 88, 425-431.
Bax, A., Clore, G.M., Driscoll, P.C., Gronenborn, A.M., Ikura, M. and Kay, L.E. (1990b) J. Magn. Reson., 87, 620-627.
Delaglio, F., Grzesiek, S., Vuister, G., Zhu, G., Pfeiffer, J. and Bax, A. (1995) J. Biomol. NMR, 6, 277-293.
Fesik, S.W., Eaton, H.L., Olejniczak, E.T. and Zuiderweg, E.R.P. (1990) J. Am. Chem. Soc., 112, 886-888.
Fiala, R., Jiang, F. and Sklenár, V. (1998) J. Biomol. NMR, 12, 373-383.
Grzesiek, S. and Bax, A. (1995) J. Biomol. NMR, 6, 335-339.
Hu, W., Kakalis, L.T., Jiang, L., Jiang, F., Ye, X. and Majumdar, A. (1998) J. Biomol. NMR, 12, 559-564.
Ikura, M., Kay, L.E. and Bax, A. (1991) J. Biomol. NMR, 1, 299-304.
Jiang, L., Majumdar, A., Hu, W., Jaishree, T.J., Xu, W. and Patel, D.J. (1999) Structure, 7, 817-827.
Johnson, B.A. and Blevins, R.A. (1994) J. Biomol. NMR, 4, 603-614.
Kay, L.E., Ikura, M. and Bax, A. (1990) J. Am. Chem. Soc., 112, 888-889.
Kolk, M.H., Wijmenga, S.S., Heus, H.A. and Hilbers, C.W. (1998a) J. Biomol. NMR, 12, 423-433.
Kolk, M.H., van der Graaf, M., Wijmenga, S.S., Pleij, C.W.A., Heus, H.A. and Hilbers, C.W. (1998b) Science, 280, 434-438.
Marino, J.P., Diener, J.L., Moore, P.B. and Griesinger, C. (1997) J. Am. Chem. Soc., 119, 7361-7366.
Marion, D., Ikura, M., Tschudin, R. and Bax, A. (1989) J. Magn. Reson., 85, 393-399.
Nikonowicz, E.P. and Pardi, A. (1993) J. Mol. Biol., 232, 1141-1156.
Olejniczak, E.T., Xu, R.X. and Fesik, S.W. (1992) J. Biomol. NMR, 2, 655-659.
Pardi, A. and Nikonowicz, E.P. (1992) J. Am. Chem. Soc., 114, 9202-9203.
Ramachandran, R., Sich, C., Grune, M., Soskic, V. and Brown, L.R. (1996) J. Biomol. NMR, 7, 251-255.
Shaka, A.J., Barker, P.B. and Freeman, R. (1985) J. Magn. Reson., 64, 547-552.
Shaka, A.J., Lee, C.J. and Pines, A. (1988) J. Magn. Reson., 77, 274-293.
Sklenár, V., Dieckmann, T., Butcher, S.E. and Feigon, J. (1998) J. Magn. Reson., 130, 119-124.
Sørensen, O.W., Eich, G.W., Levitt, M.H., Bodenhausen, G. and Ernst, R.R. (1983) Progr. NMR Spectrosc., 16, 163-192.
Varani, G., Aboul-ela, F. and Allain, F.H.T. (1996) Progr. NMR Spectrosc., 29, 51-127.
Wallis, M.G., von Ahsen, U., Schroeder, R. and Famulok, M. (1995) Chem. Biol., 2, 543-552.
Wijmenga, S.S. and van Buuren, B.N.M. (1998) Progr. NMR Spectrosc., 32, 287-387.
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Hu, W., Jiang, L. Multiple-quantum HCN-CCH-TOCSY experiment for 13C/15N labeled RNA oligonucleotides. J Biomol NMR 15, 289–293 (1999). https://doi.org/10.1023/A:1008391007013
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DOI: https://doi.org/10.1023/A:1008391007013