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Determination of sugar conformation in large RNA oligonucleotides from analysis of dipole–dipole cross correlated relaxation by solution NMR spectroscopy

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Abstract

A new experiment, the forward directed quantitative Γ-HCCH-TOCSY for the measurement of the conformation of the five-membered ribosyl unit in RNA oligonucleotides, is presented. The experiment relies on quantification of cross peak intensities caused by evolution of CH,CH-dipole–dipole cross correlated relaxation in non-evolution periods and the resolution enhancement obtainable in forward directed HCC-TOCSY transfer. Cross correlated relaxation rates are interpreted to reveal the sugar conformation of 22 out of 25 nucleotides in an isotopically labelled 25-mer RNA. The results obtained with this new method are in agreement with the conformational analysis derived from 3J(H,H) coupling constants.

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Authors and Affiliations

  1. Institut für Organische Chemie, Johann-Wolfgang-Goethe-Universität Frankfurt, Marie-Curie-Straße 11

    Christian Richter, Christian Griesinger & Isabella Felli

  2. Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, U.K.

    Paul T. Cole & Gabriele Varani

  3. Massachusetts Institute of Technology, Department of Chemistry, Francis Bitter Magnet Laboratory, 170 Albany Street, Bldg, NW14, Cambridge, MA, 02139, U.S.A.

    Harald Schwalbe

Authors
  1. Christian Richter
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  2. Christian Griesinger
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  3. Isabella Felli
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  4. Paul T. Cole
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  5. Gabriele Varani
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  6. Harald Schwalbe
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Richter, C., Griesinger, C., Felli, I. et al. Determination of sugar conformation in large RNA oligonucleotides from analysis of dipole–dipole cross correlated relaxation by solution NMR spectroscopy. J Biomol NMR 15, 241–250 (1999). https://doi.org/10.1023/A:1008319130714

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  • DOI: https://doi.org/10.1023/A:1008319130714

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