Journal of Biomolecular NMR

, Volume 11, Issue 2, pp 213–220 | Cite as

A Sensitive Pulse Scheme for Measuring the Backbone Dihedral Angle ψ Based on Cross-correlation Between 13Cα-1Hα Dipolar and Carbonyl Chemical Shift Anisotropy Relaxation Interactions

  • Daiwen Yang
  • Kevin H. Gardner
  • Lewis E. Kay


A pulse scheme for measuring cross-correlation between 13Cα-1Hα dipolar and carbonyl chemical shift anisotropy relaxation mechanisms is presented from which the protein backbone dihedral angle ψ is measured. The method offers significant sensitivity gains relative to our recently published scheme for measuring ψ based on this cross-correlation effect [Yang et al. (1997) J. Am. Chem. Soc., 119, 11938-11940]. The utility of the method is demonstrated with an application to a 42 kDa complex of 15N,13C-labeled maltose binding protein and β-cyclodextrin.

chemical shift anisotropy cross-correlation psi 


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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Daiwen Yang
    • 1
  • Kevin H. Gardner
    • 1
  • Lewis E. Kay
    • 1
  1. 1.Protein Engineering Network Centers of Excellence and Departments of Molecular and Medical Genetics, Biochemistry and ChemistryUniversity of TorontoTorontoCanada

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