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ALASKA: A Mathematica package for two-state kinetic analysis of protein folding reactions

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Abstract

A Mathematica package (ALASKA) has been developed to simplify the measurement of protein folding kinetics by analysis of 1H NMR lineshape analysis. This package reads NMR data in ASCII format and can simulate an aromatic 1 NMR spectrum with or without lineshape broadening from chemical exchange. We describe the analysis of a urea denaturation series of a fast-folding protein, the G46A/G48A variant of monomeric λ repressor.

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Authors and Affiliations

  1. Department of Biochemistry, Duke University Medical Center, Durham, NC, 27710, USA

    Rendall E. Burton, Richard S. Busby & Terrence G. Oas

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  1. Rendall E. Burton
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  2. Richard S. Busby
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  3. Terrence G. Oas
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Burton, R.E., Busby, R.S. & Oas, T.G. ALASKA: A Mathematica package for two-state kinetic analysis of protein folding reactions. J Biomol NMR 11, 355–360 (1998). https://doi.org/10.1023/A:1008276703114

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