Abstract
Hepatitis delta virus (HDV) is a satellite virus of the hepatitis B virus (HBV) which provides the surface antigen for the viral coat. Our results show that the N-terminal leucine-repeat region of hepatitis delta antigen (HDAg), encompassing residues 24–50, binds to the autolytic domain of HDV genomic RNA and attenuates its autolytic activity. The solution conformation of a synthetic peptide corresponding to residues 24–50 of HDAg as determined by two-dimensional 1H NMR and circular dichroism techniques is found to be an α-helix. The local helix content of this peptide was analyzed by NOEs and coupling constants. Mutagenesis studies indicate that Lys38, Lys39, and Lys40 within this α-helical peptide may be directly involved in RNA binding. A structural knowledge of the N-terminal leucine-repeat region of HDAg thus provides a molecular basis for understanding its role in the interaction with RNA.
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Cheng, JW., Lin, IJ., Lou, YC. et al. Local helix content and RNA-binding activity of the N-terminal leucine-repeat region of hepatitis delta antigen. J Biomol NMR 12, 183–188 (1998). https://doi.org/10.1023/A:1008270202095
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DOI: https://doi.org/10.1023/A:1008270202095