Abstract
A full length cDNA clone of grapevine virus A (GVA) was constructed downstream from the bacteriophage T7 RNA polymerase promoter. Capped in vitro-transcribed RNA was infectious in Nicotiana benthamiana and N. clevelandii plants. Symptoms induced by the RNA transcripts or by the parental virus were indistinguishable. The infectivity of the in vitro-transcribed RNA was confirmed by serological detection of the virus coat and movement proteins and by observation of virions by electron microscopy. This is the first report of infectious RNA transcripts derived from a full-length cDNA clone of a member of the Vitivirus genus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Martelli G.P., Minafra A., and Saldarelli P., Arch Virol 142, 1929±1932, 1997.
Chevalier S., Greif C., Clauzel J.M., Walter B., and Fritsch C., J Phytopathol 143, 369±373, 1995.
Credi R., Plant Dis 81, 1288±1292, 1997.
Garau R., Prota U., Piredda R., and Boscia D., Vitis 33, 161± 163, 1994.
Minafra A., Saldarelli P., and Martelli G.P., Arch Virol 142, 417±423, 1997.
Minafra A., Saldarelli P., Grieco F., and Martelli G.P., Arch Virol 137, 249±261, 1994.
Boyer J.-C. and Haenni A.-L., Virology 198, 415±426, 1994.
Steere R.L., in Smith M. and Lauffer M.A., (eds). Advances in Virus Research. Academic Press, New York, 1959, pp. 1±73.
Dodds J., in Matthews R.E.F. (ed). Diagnosis of Plant Virus Diseases. CRC Press Inc., Boca Ratonn, 1993, pp. 273±294.
Ashulin L., Mawassi M., and Bar Joseph M., Met Mol Cell Biol 3, 83±89, 1992.
Devereaux J., Heaberli P., and Smithies O., Nucleic Acids Res 12, 387±395, 1984.
Rubinson E., Galiakparov N., Radian S., Sela I., Tanne E., and Gafny R., Phytopathology 87, 1041±1045, 1997.
Colmer C.W. and Kaper J.M., Virology 145, 249±259, 1985.
Dolja V.V. and Atabekov J.A., FEBS Lett 214, 313±316, 1987.
Karasev A., Boyko V.P., Gowda S., Nikolaeva O.V., Hilf M.E., Koonin E.V., Niblett C.L., Cline K.C., Gumpf D.J., Lee R.F., Garnsey S.M., and Dawson W.O., Virology 208, 511±520, 1994.
Wengler G. and Gross H.J., Nature 282, 754±756, 1979.
Yu H.H. and Wong S.M., Arch Virol 143, 1617±1620, 1998.
Conti M., Milne G., Luisoni E., and Boccardo G., Phytopathology 79, 394±399, 1980.
Agran M.K., Di Terlizzi B., Boscia D., Minafra A., Savino V., Martelli G.P., and Askri F., Vitis 29, 43±48, 1990.
Engelbrecht D.J. and Kasdorf G.G.F., Phytopathol Medit 24, 101±105, 1985.
Engelbrecht D.J. and Kasdorf G.G.F., Phytophylactica 22, 341± 346, 1990.
Garau R., Prota V.A., Boscia D., Fiori M., and Prota U., Vitis 34, 67±68, 1995.
Rosciglione B., Castellano M.A., Martelli G.P., Savino V., and Cannizzaro G., Vitis 22, 331±347, 1983.
Rosciglione B. and Castellano M.A., Phytopathol Medit 24, 186±188, 1985.
La Notte P., Buzkan N., Choueiri E., Minafra A., and Martelli G.P., J Plant Pathol 78, 79±85, 1997
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Galiakparov, N., Tanne, E., Sela, I. et al. Infectious RNA Transcripts from Grapevine Virus A cDNA Clone. Virus Genes 19, 235–242 (1999). https://doi.org/10.1023/A:1008192831883
Issue Date:
DOI: https://doi.org/10.1023/A:1008192831883