Abstract
The separation of lactic acid from complex fermentation broth was examined. Liquid–liquid extraction using reversible chemical complexation for reactive extraction was chosen to be the separation method. Over 50% yield of lactic acid was obtained from fermented broth in a single extraction step, when using the tertiary amine as the extractant, 1-dekanol as the diluent and trimethylamine (TMA) as the stripping solution. The effect of complex media on the extraction behaviour has hardly been examined previously.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Järvinen M. Separation of lactic acid from fermented broth by reactive extraction. Diploma thesis, Oulu 1997, University of Oulu, Department of Process Engineering. 111 p.
Hano T, Matsumoto M, Uenoyama S, Ohtake T, Kawano Y. and Miura S. (1993). Separation of lactic acid from fermented broth by solvent extraction. Bioseparation 3: 321-326.
Kertes AS and King CJ (1986). Extraction chemistry of fermentation product carboxylic acids. Biotechnol. Bioeng. 28,(2): 269-282.
San Martin M, Pazos C and Coca J (1992). Reactive extraction of lactic acid with Alamine 336 in the presence of salts and lactose. J. Chem. Technol. Biotechnol. 54: 1-6.
Siebold M, Frieling P, Joppien R, Rindfleisch D, Schügerl K. and Röper H. (1995). Comparison of the production of lactic acid by three different lactobacilli and its recovery by extraction and electrodialysis. Process Biochem. 30:(1): 81-95.
Tamada JA and King CJ (1990). Extraction of carboxylic acids with amine extractants. 2. Chemical interactions and interpretation of data. Industrial & Engineer. Chem. Res. 29(7): 1327-1332.
Tamada JA and King CJ (1990). Extraction of carboxylic acids with amine extractants. 3. Effect of temperature, water coextraction, and process considerations. Industrial & Engineer. Chem. Res. 29(7): 1333-1338.
Tamada JA, Kertes AS and King CJ (1990). Extraction of carboxylic acids with amine extractants. 1. Equilibria and law of mass action modelling. Industrial & Engineer. Chem. Res. 29(7): 1319-1327.
Tung LA and King CJ (1991). Recovery of carboxylic acids using extractants that are reactive and regenerable at pH > pK a1. First Separations Division Topical Conference on Separations Technologies: New developments and opportunities. Miami Beach. American Institute of Chemical Engineers, pp. 1147-1151.
Yang S-T White S and Hsu S-T (1991). Extraction of carboxylic acids with tertiary and quaternary amines: Effect of pH. Industrial & Engineer. Chem. Res. 30(6): 1335-1342.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Järvinen, M., Myllykoski, L., Keiski, R. et al. Separation of lactic acid from fermented broth by reactive extraction. Bioseparation 9, 163–166 (2000). https://doi.org/10.1023/A:1008183322075
Issue Date:
DOI: https://doi.org/10.1023/A:1008183322075