Skip to main content

Advertisement

SpringerLink
Log in

Identification of a Functionally Important Amino Acid Residue Near to the Amino-Terminus of the Human Immunodeficiency Virus Type 1 Vif Protein

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Previous studies have reported the importance of residues in the central and carboxy-terminal regions of HIV-1 Vif as being important for a Vif+ phenotype. Mutants in a residue (Trp21) near to the amino-terminus of Vif, which is conserved between HIV-1, HIV-2, and SIVmac, were generated in the permissive C8166 cell line, and their Vif phenotype determined by monitoring their growth on the nonpermissive H9 cell line. Of seven variants at position 21 of Vif only phenylalanine substituted successfully for Trp21. The expression of the mutant Vif proteins was shown to be similar to wildtype levels, in C8166 cells, in all cases except one. These data identify Trp21 as an amino-terminal residue which is important to Vif function, in allowing growth of virus in nonpermissive H9 cells, and suggest that there is limited scope for variation at this position of HIV-1 Vif.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sonigo P., Alizon A., Staskus K., Klatzmann D., Cole S., Danos O., Retzel E., Tiollais P., Haase A., and Wain-Hobson S.,Cell 42, 369–382, 1985.

    Google Scholar 

  2. Talbott R.L., Sparger E.E., Lovelace K.M., Fitch W.M., Pederson N.C., Luciw P.A., and Elder J.H., Proc Natl Acad Sci USA 86, 5743–5747, 1989.

    Google Scholar 

  3. Chakrabati L., Guyader M., Alizon M., Daniel M.D., Desrosiers R.C., Tiollais P., and Sonigo P., Nature 328, 543–547, 1987.

    Google Scholar 

  4. Guyader M., Emerman M., Sonigo P., Clavel F., Montagnier L., and Alizon M., Nature 326, 662–669, 1987.

    Google Scholar 

  5. Garvey K.J., Oberste M.S., Elser J.E., Braun M.J., and Gonda M.A., Virology 175, 391–409, 1990.

    Google Scholar 

  6. Saltarelli M., Querat G., Konings D.A.M., Vigne R., and Clements J.E., Virology 179, 347–364, 1990.

    Google Scholar 

  7. Oberste M.S. and Gonda M.A., Virus Genes 6, 95–102, 1992.

    Google Scholar 

  8. Kawakami T., Sherman L., Dahlberg J., Gazit A., Yaniv A., Tronick S.R., and Aaronson S.A., Virology 158, 300–312, 1987.

    Google Scholar 

  9. Gabuzda D., Lawrence K., Langhoff E., Terwilliger E., Dorfman T., Hasteltine W., and Sodroski J., J Virol 66, 6489–6495, 1992.

    Google Scholar 

  10. Sakai H., Shibata R., Sakuragi J.-I., Sakuragi S., KawamuraM., and Adachi A., J Virol 67, 1663–1666, 1993.

    Google Scholar 

  11. von Schwedler U., Song J., Aiken C., and Trono D., J Virol 67 4945–4955, 1993.

    Google Scholar 

  12. Chowdhury I.H., Chao W., Potash M.J., Sova P., Gendelman H.E., and Volsky D.J., J Virol 70, 5336–5345, 1996.

    Google Scholar 

  13. Courcoul M., Patience C., Rey F., Blanc D., Harmache A., Sire J., Vigne R., and Spire B., J Virol 69, 2068–2074, 1995.

    Google Scholar 

  14. Gabuzda D.H., Li H., Lawrence K., Vasir B.S., Crawford K., and Langhoff E., J Acq Imm De®c Syndr 7, 908–915, 1994.

    Google Scholar 

  15. Michaels F.H., Hattori N., Gallo R.C., and Franchini G., AIDS Res Hum Retroviruses 9, 1025–1030, 1993.

    Google Scholar 

  16. Simm M., Shahabuddin M., Chao W., Allan J.S., and Volsky D.J., J Virol 69, 4582–4586, 1995.

    Google Scholar 

  17. Sodroski J., GohW.C., Rosen C., Tartar A., Portetelle D., Burny A., and Hasteltine W., Science] 231, 1549–1553, 198

    Google Scholar 

  18. Fischer A.G., Ensoli B., Ivanoff L., Chamberlain M., Petteway S., Ratner L., Gallo R.C., andWong-Staal F., Science 237, 888–893, 1987.

    Google Scholar 

  19. Strebel K., Daugherty D., Clouse K., Cohen D., Folks T., and Martin M.A., Nature 328, 728–730, 1987.

    Google Scholar 

  20. Borman A., Quillent M.C., Charneau P., Dauguet C., and Clavel F., J Virol 69, 2058–2067, 1995.

    Google Scholar 

  21. Nagashunmugam T. and Friedman H.M., DNA Cell Biol 15, 353–361, 1996.

    Google Scholar 

  22. Goncalves J., Korin Y., Zack J., and Gabuzda D., J Virol 70, 8701–8709, 1996.

    Google Scholar 

  23. Nascimbeni M., Bouyac M., Rey F., Spire B., and Clavel F., J Gen Virol 79, 1945–1950, 1998.

    Google Scholar 

  24. Sova P., and Volsky D.J., J Virol 67, 6322–6326, 1993.

    Google Scholar 

  25. Simon J.H.M. and Malim M.H., J Virol 70, 5297–5305,1996.

    Google Scholar 

  26. Camaur D. and Trono D., J Virol 70, 6106–6111, 1996.

    Google Scholar 

  27. Liu H., Wu X., Newman M., Shaw G.M., Hahn B., and Kappes J.C., J Virol 69, 7630–7638, 1995.

    Google Scholar 

  28. Fouchier R.A.M., Simon J.H.M., Jaffe A.B., and Malim M.H., J Virol 70, 8263–8269, 1996.

    Google Scholar 

  29. Karczewski M.K. and Strebel K., J Virol 70, 494–507, 1996.

    Google Scholar 

  30. Simon J.H.M., Miller D.L., Fouchier R.A.M., and Malim M.H., Virology 248, 182–187, 1998.

    Google Scholar 

  31. HoÈglund S., Öhagen, Å., Lawrence T., and Gabuzda D., Virology 201, 349–355, 1994.

    Google Scholar 

  32. Bouyac M., Rey F., Nascimbeni M., Courcoul M., Sire J., Blanc D., Clavel F., Vigne R., and Spire B., J Virol 71, 2473–2477, 1997.

    Google Scholar 

  33. Kotler M., Simm M., Zhao S., Sova P., Chao W., Ohnona S.-F., Roller R., Krachmarov C., Potash M.J., and Volsky D.J., J Virol 71, 5774–5781, 1997.

    Google Scholar 

  34. Ma X.-Y., Sova P., ChaoW., and Volsky D.J., J Virol 68, 1714–1720, 1994.

    Google Scholar 

  35. Goncalves J., Jallepalli P., and Gabuzda D.H., J Virol 68, 704–712, 1994.

    Google Scholar 

  36. Goncalves J., Shi B., Yang X., and Gabuzda D.H., J Virol 69, 7196–7204, 1995.

    Google Scholar 

  37. Bouyac M., Courcoul M., Bertoia G., Baudat Y., Gabuzda D., Blanc D., Chazal N., Boulanger P., Sire J., Vigne R., and Spire B., J Virol 71, 9358–9365, 1997.

    Google Scholar 

  38. Yang X., Goncalves J., and Gabuzda D., J Biol Chem 271, 10121–10129, 1996.

    Google Scholar 

  39. Huvent I., Hong S.S., Fournier C., Gay B., Tournier J., CarrieÁre C., Courcoul M., Vigne R., Spire B., and Boulanger P., J Gen Virol 79, 1069–1081, 1998.

    Google Scholar 

  40. Simon J.H.M., Southerling T.E., Peterson J.C., Meyer B.E., and Malim M.H., J Virol 69, 4166–4172, 1995.

    Google Scholar 

  41. Simon J.H.M., Miller D.L., Fouchier R.A.M., Soares M.A., Peden W.C., and Malim M.H., EMBO J 17, 1259–1267, 1998.

    Google Scholar 

  42. Yamamoto Y., Saito Y., Iida S., Asano J., Sone S., and Adachi A., Virus Genes 14, 195–200, 1997.

    Google Scholar 

  43. Reddy T.R., Kraus G., Yamada O., Looney D.J., Suhasini M., and Wong-Staal F., J Virol 69, 3549–3553, 1995.

    Google Scholar 

  44. Hubert J.J., Bioassay. Kendall/Hunt Publishing Company, Toronto, 1980, p. 62.

    Google Scholar 

  45. Chang S.L., Berg G., Busch K.A., Stevenson R.E., Clarke N.A., and Kabler P.W., Virology 6, 27–42, 1958.

    Google Scholar 

  46. Wieland U., Seelhoff A., Hofmann A., KuÈhn J.E., Eggers H.J., Mugyenyi P., and Schwander S., J Gen Virol 78, 393–400, 1997.

    Google Scholar 

  47. Myers G., Berzofsky J.A., Korber B., Smith R.F., and Pavlakis G.N., ``A compilation and analysis of nucleic acid and amino acid sequences''. Theoretical biology and biophysics group T-10, Los Alamos National Library, Los Alamos, 1993, p. II-A-

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, University of Warwick, Coventry, England, CV4 7AL

    Mark Boyce, Peter Willingmann & Malcolm McCrae

Authors
  1. Mark Boyce
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter Willingmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Malcolm McCrae
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Malcolm McCrae.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boyce, M., Willingmann, P. & McCrae, M. Identification of a Functionally Important Amino Acid Residue Near to the Amino-Terminus of the Human Immunodeficiency Virus Type 1 Vif Protein. Virus Genes 19, 15–22 (1999). https://doi.org/10.1023/A:1008180421245

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008180421245

Search

Navigation