Abstract
The nucleotide sequence of two cloned restriction fragments encompassing the granulin genes from the granuloviruses of the potato tuber moth, Phthorimaea operculella, PhopGV, and the Egyptian cotton leaf worm, Spodoptera littoralis, SpliGV, have been determined.
Although both viruses are able to infect the same Ph. operculella cell line, their granulins do not cluster in the same phylogenetic branches. PhopGV ganulin is closely related to Cydia pomonella GV (CpGV) and Cryptophlebia leucotreta GV (ClGV) (95.2 and 94% identity at the aminoacid level), while SpliGV granulin falls close to Trichoplusia ni GV and Xestia c-nigrum GV (91.6 and 92.0% respectively).
The gene organization around the granulins reflects this clustering. Upstream the PhopGV granulin, an ORF belonging to the ME53 gene family (as ORF 124R of CpGV and 909 of ClGV) is present, while no equivalent ORF is found in this region in SpliGV. Downstream the granulin, both viruses present a gene homologous to the Autographa californica nucleopolyhedrovirus (AcMNPV) ORF 9 followed by a Protein Kinase (AcMNPV ORF10). The structure of this region seems thus conserved not only among nucleopolyhedroviruses but also in at least some granuloviruses.
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Yearian W.C. and Young S.Y., In Kustak, E. (ed). Microbial and Viral Pesticides. Marcel Dekker, Inc, New York, 1982, pp. 387-423.
Winstanley D. and Crook N.E., J Gen Virol 74, 1599–1609, 1993.
Léry X., Giannotti J., Taha A., Ravallec M., and Abol-Ela S., In Vitro Cell Dev Biol 33, 640–646, 1997.
Léry X., Giannotti J., and Abol-Ela S., Acta Virol 42, 13–21, 1998.
Léry X., Khamiss O., Nour-El-Din A., Giannotti J., and Abol-Ela S., Acta Virol 41, 169–174, 1997.
Southern E.M., J Mol Biol 98, 503–517, 1975.
Pearson W.R. and Lipman D.J., Proc Natl Acad Sci USA 85, 2444–2448, 1988.
Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J., J Mol Biol 215, 403–410, 1990.
Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., and Higgins D.G., Nucleic Acids Research 25, 4876–4882, 1997.
Saitou N. and Nei M., Mol Biol Evol 4, 406–425, 1987.
Perrière G. and Gouy M., Biochimie 78, 364–369, 1996.
Page R.D.M., Comput Applic Biosci 12, 357–358, 1996.
Arella M., Lavallée C., Belloncik S., and Furuichi Y., J Virol 62, 211–217, 1988.
Zanotto P.M.A., Kessing B.D., and Maruniak J.E., J Invertebr Pathol 62, 147–164, 1993.
Crook N.E., James J.D., Smith I.R., and Winstanley D., J Gen Virol 78, 965–974, 1997.
Bulach D.M., Kumar C.A., Zaia A., Liang B., and Tribe D.E., J Invertebr Pathol 73, 59–73, 1999.
Jehle J.A. and Backhaus H., J Gen Virol 75, 3667–3671, 1994.
Knebel-Mörsdorf D., Kremer A., and Jahnel F., J Virol 67, 753–758, 1993.
Goto C., Hayakawa T., and Maeda S., Virus genes 16, 199–210, 1998.
Hayakawa T., Ko R., Okano K., Seong S-I., Goto C., and Maeda S., Virology 262, 277–297, 1999.
Kuzio J., Pearson M.N., Harwood S.H., Funk C.J., Evans J.T., Slavicek J.M., and Rohrmann G.F., Virology 253, 17–34, 1999.
Vialard J.E. and Richardson C.D., J Virol 67, 5859–5866, 1993.
Hu Z.H., Arif B.M., Jin F., Martens J.W., Chen X.W., Sun J.S., Zuidema D., Goldbach R.W., and Vlak J.M., J Gen Virol 79, 2841–2851, 1998.
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Taha, A., Nour-el-Din, A., Croizier, L. et al. Comparative Analysis of the Granulin Regions of the Phthorimaea operculella and Spodoptera littoralis Granuloviruses. Virus Genes 21, 147–155 (2000). https://doi.org/10.1023/A:1008179228236
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DOI: https://doi.org/10.1023/A:1008179228236