Antioxidant activities of phycocyanobilin prepared from Spirulina platensis

Abstract

The antioxidative activity of phycocyanobilin fromSpirulina platensis was evaluated againstoxidation of methyl linoleate in a hydrophobic systemor with phosphatidylcholine liposomes. Phycocyanobilin as well as phytochemicals includingα-tocopherol, caffeic acid and zeaxanthin,effectively inhibited the peroxidation of methyllinoleate and produced a prolonged induction period.Oxidation of phosphatidylcholine liposomes was alsocontrolled markedly by adding phycocyanobilin orα-tocopherol. Phycocyanobilin was distributedoutside in the liposomes to scavenge radicals fromAAPH and to prevent initiation of radical chainreactions. When the concentrations of phycocyanin andphycocyanobilin in the reaction mixture were adjustedequally on a phycocyanobilin basis, the activity ofphycocyanobilin was almost the same as that ofphycocyanin in the AAPH-containing reaction mixture.The antioxidizing action of phycocyanin prepared fromspray-dried Spirulina almost agreed with thatfrom fresh Spirulina in the AAPH-containingreaction mixture. These results suggest thatphycocyanobilin is responsible for the majority of theantioxidative activity of phycocyanin and may act asan effective antioxidant in a living human body.

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Hirata, T., Tanaka, M., Ooike, M. et al. Antioxidant activities of phycocyanobilin prepared from Spirulina platensis . Journal of Applied Phycology 12, 435–439 (2000). https://doi.org/10.1023/A:1008175217194

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  • active oxygen
  • blue-green alga
  • cyanobacteria
  • liposome
  • oxidation
  • phycobilin
  • phycobiliprotein
  • phycocyanin
  • radical