Abstract
Recombinant plasmids that express human foamy virus (HFV) Bel 1 transactivator and human estrogen receptor (ER) fusion proteins were constructed. The HFV bel 1 gene was inserted up- and downstream of the ER gene. Recombinant Bel 1-ER and ER-Bel 1 fusion proteins were expressed in eukaryotic cells. In the absence of estrogen, the ER moiety of the fusion proteins suppressed Bel 1-mediated transactivation as measured in CAT reporter gene-based transactivation assays. However, transactivation of the HFV LTR and the HFV internal promoter by Bel 1-ER and ER-Bel 1 fusion proteins was recovered in the presence of estrogen. Thus, the transactivation function of the Bel 1 moiety of the chimeric Bel 1-ER fusion proteins can be efficiently, specifically, and intentionally activated and inactivated by simply adding the low-molecular weight effector estrogen.
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Yang, P., Flu¨gel, R.M., Li, W. et al. Human Foamy Virus Bel 1 Transactivator/Estrogen Receptor Fusion Proteins Allow Inducible Transactivation of Both Human Foamy Virus Promoters. Virus Genes 21, 209–213 (2000). https://doi.org/10.1023/A:1008143615032
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DOI: https://doi.org/10.1023/A:1008143615032