Abstract
The South African Territories (SAT) types of foot-and-mouth disease (FMD) virus show marked genomic and antigenic variation in sub-Saharan Africa that is to a large extent geographically determined. This has implications for selection of appropriate vaccine strains as well as the accuracy of laboratory diagnosis. However, adaptation of field isolates as vaccine strains is cumbersome, time consuming and expensive. We propose the construction of recombinant viruses in which specific antigenic determinants can be manipulated. To achieve this goal, the structural-protein-coding region of a SAT 2 vaccine strain, ZIM 7/83/2, was determined and compared with two other known SAT 2 P1 regions. Five hypervariable regions were identified of which four are situated within VP1. The cleavage sites for proteolytic processing differs from serotype A, while the junction between P1/2A is variable within the SAT 2 serotype. These differences could influence the construction of recombinant vaccines.
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References
Berinstein A., Roivainen M., Hovi T., Mason P.W., and Baxt B., J Virol 69, 2664±2666, 1995.
Belsham G.J., Prog Biophys Molec Biol 60, 241±260, 1993.
Baxt B., Vakharia V., Moore D.M., Franke A.J., and Morgan D.O., J Virol 63, 2143±2151, 1989.
Crowther J.R., Rowe C.A., and Butcher R., Epidemiol Infect 111, 391±406, 1993.
Harley E.H., ``DAPSA ± a program for DNA and protein sequence analysis, version 4.31'', Department of Chemical Pathology, University of Cape Town, South Africa, 1998.
Hunter P., OIE Sci Tech Rev 15, 913±922, 1996.
Kitson J.D.A., McCahon D., and Belsham G., Virology 179, 26±34, 1990.
Mateu M.G., Martinez M.A., Capucci L., Andreu D., Giralt E., Sobrino F., Brocchi E., and Domingo E., J Gen Virol 71, 629± 637, 1990.
Mateu M.G., Virus Res 38, 1±24, 1995.
Palmenberg A., in Semler B.L. and Ehrenfeld E. (eds.) Molecular Aspects of Picornavirus Infection and Detection. ASM, Washington, DC, p. 211, 1989.
Rico-Hesse R., Pallansch M.A., Nottay B.K., Virology 160, 311±322, 1987.
Robertson B.H., Grubman M.J., Weddell G.N., Moore D.M., Welsh J.D., Fischer T., Dowbenko D.J., Yansura D.G., Small B., and Klein D.G.J., Virol 54, 651±660, 1985.
Sa-Carvalho D., Rieder E., Baxt B., Rodarte R., Tanuri T., and Mason P.W., J Virol 71, 5115±5123, 1997.
Thomson G.R. in Coetzer J.A.W., Thomson G.R., and Tustin R.C. (eds) Infectious Diseases of Livestock with Special Reference to Southern Africa. Oxford University Press, Cape Town, p. 825, 1994.
VoslooW., Kirkbride E., Bengis R.G., Keet A.F., and Thomson G.R., Epidemiol Infect 114, 203±218, 1995.
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Rensburg, H.G.V., Nel, L.H. Characterization of the Structural-Protein-Coding Region of SAT 2 Type Foot-and-Mouth Disease Virus. Virus Genes 19, 229–233 (1999). https://doi.org/10.1023/A:1008140815045
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DOI: https://doi.org/10.1023/A:1008140815045