Inhibitory effect of phycocyanin from Spirulina platensis on the growth of human leukemia K562 cells

Abstract

The effect of phycocyanin from Spirulinaplatensis on the growth of human chronic myelogenousleukemia-blast crisis K562 cells was studied bysemi-solid agar assay and cell viability measurement. Phycocyanin significantly inhibited the growth of K562cells in a dose-dependent manner. The IC50 value ofthe phycocyanin was 72.5 mg L-1. After the K562cells were cultured with phycocyanin for 6 days, flowcytometric assays showed that more K562 cells wereblocked to progress through S-phase and arrested at G1phase. DNA fragmentation assay indicated that therewas no ladder of DNA fragments of approximately 200-bpmultiples, indicating that apoptosis had not occurred. Western blot analysis showed that Bcl-2 protein wasexpressed, but its level remained unchanged, whereasthe expression level of c-myc increased. Thesefindings suggest that phycocyanin may be able toinhibit the growth of K562 cells by pathways otherthan apoptosis, and that changed a expression patternof the c-myc protein may be involved in such inhibition.

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References

  1. Arriola EL, Rodriguez-Lopez AM, Hickman JA, Chresta CM (1999) Bcl-2 overexpression results in reciprocal downregulation of Bcl-XL and sensitizes human testicular germ cell tumors to chemotherapy-induced apoptosis. Oncogene 18: 1457-1464.

    Google Scholar 

  2. Belay A, Yoshimichi O, Kazuyuki M, Hidenori S (1993) Current knowledge on potential health benefits of Spirulina. J. appl. Phycol. 5: 235-241.

    Google Scholar 

  3. Brejc K, Ficner R, Huber R, Steinbacher S (1995) Isolation, crystallization, crystal structure analysis and refinement of allophycocyanin from the cyanobacterium Spirulina platensis at 2.3 Å resolution. J. mol. Biol. 249: 424-440.

    Google Scholar 

  4. Canelles M, Delgado MD, Hyland KM, Lerga A, Richard C, Dang CV, Leon J (1997) Max and inhibitory c-myc mutants induce erythroid differentiation and resistance to apoptosis in human myeloid leukemia cells. Oncogene 14: 1315-1327.

    Google Scholar 

  5. Chang ST, Yung BYM (1996) Potentiation of sodium butyrate-induced apoptosis by vanadate in human promyelocytic leukemia cells line HL-60. Biochem. biophys. Res. Comm. 221: 594-601.

    Google Scholar 

  6. Dainippon Ink & Chemical Co. (DIC) (1983) Antitumoral agents containing phycobilin. Japanese Patent # 58-65216, inventors: N. Iijima, N. Fujii & H. Shimamatsu; assignees: Dainippon Ink & Chemicals & Tokyo Stress Foundation, April 18, 6 pp.

  7. Harrington EA, Fanidi A, Evan GI (1994) Oncogenes and cell death (review) (112 refs). Curr. Op. Genet. Develop. 4 (1): 120-129 (February).

    Google Scholar 

  8. Huang DC, O'Reilly LA, Strasser A, Cory S (1997) The antiapoptosis function of Bcl-2 can be genetically separated from its inhibitory effect on cell cycle entry. EMBO J. 16(15): 4628-4638.

    Google Scholar 

  9. Luo D, Cheng SCS, Xie Y (1999) Expression of Bcl-2 family proteins during chemotherapeutic agents-induced apoptosis in the hepatoblastoma HepG2 cell line. Br. J. biomed. Sci. 56: 114-122.

    Google Scholar 

  10. Marcu KB, Bossone SA, Patel AJ (1992) Myc function and regulation (review) (432 refs). Annu. Rev. Biochem. 61: 809-60.

    Google Scholar 

  11. Morcos NC, Berns M, Henry WL (1988) Phycocyanin: laser activation, cytotoxic effect, and uptake in human atherosclerotic plaque. Lasers Surg. Med. 8: 10-17.

    Google Scholar 

  12. Noda H, Amano H, Arashima K, Hashimoto S, Nishizawa K (1989) Studies on the antitumor activity of marine algae. Bull. Jap. Soc. Sci. Fish. 55: 1254-1264.

    Google Scholar 

  13. Ray S, Bullock G, Nunez G, Tang C, Ibrado AM, Huang Y, Bhalla K (1996) Enforced expression of Bcl-Xs induces differentiation and sensitizes chronic myelogenous leukemia-blast crisis K562 cells to 1-beta-D-arabinofuranosylcytosine-mediated differentiation and apoptosis. Cell Growth & Differentiation 7(12): 1617-1623.

    Google Scholar 

  14. Riou D, Colliec-Jouaults S, Pinczon du Sel D, Bosch S, Siavoshian S, Le Bert V, Tomasoni C, Sinquin C, Durand P, Roussakis C (1996) Antitumor and antiproliferative effects of a fucan extracted from ascophyllum nodosum against a non-small-cell bronchopulmonary carcinoma line. Anticancer Res. 16(3A): 1213-1218.

    Google Scholar 

  15. Romay C, Armesto J, Remirez D, Gonzalez R, Ledon N, Garcis I (1998) Antioxidant and anti-inflammatory properties of C-phycocyanin from blue-green algae. Inflammation Res. 47 (1): 36-41.

    Google Scholar 

  16. Sadanori M, Hiroyoshi M, Kyoko N, Fuminori K (1993) Effect of seaweed preparations on murine immunocytes. J. appl. Phycol. 5: 629-637.

    Google Scholar 

  17. Schwartz JL, Sklar G, Reid S, Trickler D (1988) Prevention of experimental oral cancer by extracts of Spirulina dunaliella algae. Nutr. Cancer 11: 127-134.

    Google Scholar 

  18. Vadiraja BB, Gaikwad NW, Madyastha KM (1998) Hepatoprotective effect of C-phycocyanin: protection for carbon tetrachloride and R-(+)-pulegone-mediated hepatotoxicity in rats. Biochem. biophys. Res. Comm. 249(2): 428-431.

    Google Scholar 

  19. Zhang CW, Tseng CT, Zhang YZ (1996) Purification and physiochemical properties of phycobiliprotein of Spirulina Platensis var Nanjingensis. Natural Product Research and Development 8(2): 29-34.

    Google Scholar 

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Liu, Y., Xu, L., Cheng, N. et al. Inhibitory effect of phycocyanin from Spirulina platensis on the growth of human leukemia K562 cells. Journal of Applied Phycology 12, 125–130 (2000). https://doi.org/10.1023/A:1008132210772

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  • Bcl-2
  • C-myc
  • growth inhibition
  • G1 arrest
  • K562 cells
  • Phycocyanin
  • Spirulina platensis