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Complete Nucleotide Sequence of the Rice Tungro Spherical Virus Genome of the Highly Virulent Strain Vt6

Abstract

The complete nucleotide sequence of rice tungro spherical virus (RTSV) strain Vt6, originally from Mindanao, the Philippines, with higher virulence to resistant rice cultivars, was determined and compared with the published sequence for the Philippine-type strain A (RTSV-A-Shen). It was reported that RTSV-A was not able to infect a rice resistant cultivar TKM 6 (10). RTSV-Vt6 and RTSV-A-Shen share 90% and 95% homology at nucleotide and amino-acid levels, respectively. The N-terminal leader sequence of RTSV-Vt6 contained a 39-amino acids-region (positions 65 to 103) which was totally different from that of RTSV-A-Shen; the difference resulted from frame shifting by nucleotide insertions and deletions. To confirm the amino-acid sequence differences of the leader polypeptide, the same region was cloned and sequenced using a newly obtained variant of RTSV-type 6, which had been collected in the field of IRRI, and seven field isolates from Mindanao, the Philippines. Since all the sequences of the target region are identical to that of the Vt6 leader polypeptide, the sequence difference in the leader region seems not to correlate with the virulence of Vt6.

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References

  1. 1.

    Saito Y., Trop Agric Res Ser No 10, 129-135, 1977.

    Google Scholar 

  2. 2.

    Hibino H., Roechan M., and Sudarisman S., Phytopathology 68, 1412-1416, 1978.

    Google Scholar 

  3. 3.

    Hibino H., Saleh N., and Roechan M., Phytopathology 69, 1266-1268, 1979.

    Google Scholar 

  4. 4.

    Hibino H., Ann Phytopathol Soc Jpn 49, 545-553, 1983.

    Google Scholar 

  5. 5.

    Murphy F.A., Fauquet C.M., Bishop D.H.L., Ghebrial S.A., Jarvis A.W., Martelli G.P., Mayo M.A., and Summers M.D., Virus taxonomy. Springer-Verlag, Wien/New York, pp. 339-340, 1995.

    Google Scholar 

  6. 6.

    Hibino H., Ishikawa K., Omura T., Cabauatan P.Q., and Koganezawa H., Phytopathology 81, 1130-1132, 1991.

    Google Scholar 

  7. 7.

    Jones M.C., Gough K., Dasgupta I., Rao S., and Cliffe J., J Gen Virol 72, 757-761, 1991.

    Google Scholar 

  8. 8.

    Shen P., Kaniewska M., Smith C., and Beachy R.N., Virology 193, 621-630, 1993.

    Google Scholar 

  9. 9.

    Thole V. and Hull R., Virus Genes 13, 239-246, 1996.

    Google Scholar 

  10. 10.

    Cabauatan P.Q., Cabunagan R.C., and Koganezawa H., Phytopathology 85, 77-81, 1995.

    Google Scholar 

  11. 11.

    Cabauatan P.Q. and Hibino H., Plant Dis 72, 526-528, 1988.

    Google Scholar 

  12. 12.

    Druka A., Burns T., Zhang A., and Hull R., J Gen Virol 77, 1975-1983, 1996.

    Google Scholar 

  13. 13.

    Thole V. and Hull R., Virology 247, 106-114, 1998.

    Google Scholar 

  14. 14.

    Reddick B.B., Habera L.F., and Law M.D., J Gen Virol 78, 1165-1174, 1997.

    Google Scholar 

  15. 15.

    DawsonW.O. and Hilf M.E., Annu Rev Plant Physiol Plant Mol Biol 43, 527-555, 1992.

    Google Scholar 

Download references

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Isogai, M., Cabauatan, P.Q., Masuta, C. et al. Complete Nucleotide Sequence of the Rice Tungro Spherical Virus Genome of the Highly Virulent Strain Vt6. Virus Genes 20, 79–85 (2000). https://doi.org/10.1023/A:1008116408733

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  • rice tungro disease
  • Sequiviridae
  • Waikavirus
  • virulent strain
  • complete sequence