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Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization

Abstract

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1- bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml-1 to 400 ngml-1 of ginsenoside Rb1.

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Correspondence to Yukihiro Shoyama.

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Tanaka, H., Fukuda, N. & Shoyama, Y. Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization. Cytotechnology 29, 115–120 (1999). https://doi.org/10.1023/A:1008068718392

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  • DOI: https://doi.org/10.1023/A:1008068718392

  • ginsenoside-Rb1
  • ELISA
  • monoclonal antibody
  • qualitative analysis
  • mass spectrometry