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Anti-Herpes Simplex Virus substances produced by the marine green alga, Dunaliella primolecta

Journal of Applied Phycology volume 10, pages 349–356 (1998)Cite this article

Abstract

Among 106 microalgae tested, the cytopathic effect (CPE) upon Vero cells of herpes simplex virus, Type 1 (HSV-1) was inhibited by four methanol extracts of Dunaliella bioculata C-523, D. primolecta C-525, Lyngbya sp. M-9 and Lyngbya aerugineo-coerulea M-12. The green alga, D. primolecta, had the highest anti HSV-1 activity, since 10 μg mL-1 of extract from this alga completely inhibited the CPE. This activity was similar to that of acyclovir at the same concentration. We compared anti-viral activities against adeno virus, herpes simplex virus-2 (HSV-2), Japanese Encephalitis and Polio viruses. Only the CPE of HSV-2 was inhibited. Thus, the factor was specific against HSV. The antiviral activity was apparently excited during HSV adsorption and invasion of the cells. We optimized the conditions for anti HSV-1 activity by prolonging the exposure of HSV-1 to the extract. After 2 h, the CPE of even a high titer of HSV-1 (106 TCID50/0.1 mL) was completely inactivated. By use of various chromatographic techniques, three green substances having anti-HSV activity were purified from the algal mass of D. primolecta, and 5 μg mL-1 of this purified substances completely inhibited the CPE. From the analysis of NMR and MS, the chemical structures of the active substances were identified as pheophorbide-like compounds.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Setsunan University, 45-1 Nagaotoge-cho, Hirakata, Osaka, 573-01, Japan

    Souichi Ohta, Futoshi Ono, Yasuki Shiomi, Teruyuki Nakao, Osamu Aozasa, Shoji Yamaguchi, Masatoshi Nishi & Hideaki Miyata

  2. Research Center, Taisho Pharmaceutical Co., Ltd, 1-403 Yoshino-cho, Ohmiya, Saitama, 330, Japan

    Takatoshi Nagate & Kunihiro Kitamura

Authors
  1. Souichi Ohta
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  2. Futoshi Ono
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  3. Yasuki Shiomi
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  4. Teruyuki Nakao
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  5. Osamu Aozasa
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  6. Takatoshi Nagate
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  7. Kunihiro Kitamura
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  8. Shoji Yamaguchi
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  9. Masatoshi Nishi
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  10. Hideaki Miyata
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Ohta, S., Ono, F., Shiomi, Y. et al. Anti-Herpes Simplex Virus substances produced by the marine green alga, Dunaliella primolecta. Journal of Applied Phycology 10, 349–356 (1998). https://doi.org/10.1023/A:1008065226194

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  • DOI: https://doi.org/10.1023/A:1008065226194

  • algae
  • Anti-HSV substance
  • Dunaliella
  • Lyngbya sp.
  • microalgae
  • anti-virus
  • Herpes simplex virus
  • commercial microorganisms