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The Cauliflower Mosaic Virus Capsid Protein: Assembly and Nucleic Acid Binding In Vitro

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Abstract

The capsid protein of the cauliflower mosaic virus (CaMV) was expressed in a bacterial system to study CaMV assembly. Bacterial lysates contained soluble particulate material and insoluble inclusion bodies that were both used for analysis. In vitro renaturation of pIV derivatives lead to the appearance of folded sheets or large tubular structures in electron microscopy. The region between amino acid positions 77 and 332 is sufficient for self-aggregation of pIV in vitro. C-terminal deletion to amino acid position 265 still allowed dimerization but prevented further aggregation. Nucleic acid binding assays of immobilized pIV derivatives demonstrated that a region located upstream of the retroviral “zinc finger-like” motif is involved in unspecific binding dsDNA, ssDNA and RNA.

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References

  1. Hull R., Sem Virol 3, 373-382, 1992.

    Google Scholar 

  2. Rothnie H.M., Chapdelaine Y., and Hohn T., Adv Virus Res 44, 1-67, 1994.

    PubMed  CAS  Google Scholar 

  3. Torbet J., Timmins P.A., and Lvov Y., Virology 155, 721-725, 1986.

    Article  CAS  PubMed  Google Scholar 

  4. Kruse J., Timmins P., and Witz J., Virology 159, 166-168, 1987.

    Article  CAS  PubMed  Google Scholar 

  5. Cheng R.H., Olson N.H., and Baker T.S., Virology 186, 655- 668, 1992.

    Article  PubMed  CAS  Google Scholar 

  6. Al Ani R., Pfeiffer P., Lebeurier G., and Hirth L., Virology 93, 175-187, 1979a.

    Article  PubMed  CAS  Google Scholar 

  7. Shepherd R.J., Ann Rev Plant Physiol 30, 405-423, 1979.

    Article  CAS  Google Scholar 

  8. Hull R., Covey S.N., and Maule A.J., J Cell Sci Suppl. 7, 213- 229, 1987.

    CAS  Google Scholar 

  9. Marsh L.E. and Guilfoyle T.J., Virology 161, 129-137, 1987.

    Article  CAS  PubMed  Google Scholar 

  10. Mazzolini L., Dabos P., Constantin S., and Yot P., J Gen Virol 70, 3439-3449, 1989.

    CAS  Google Scholar 

  11. Himmelbach A., Chapdelaine Y., and Hohn T., Virology 217, 147-157, 1996.

    Article  PubMed  CAS  Google Scholar 

  12. Al Ani R., Pfeiffer P., and Lebeurier G., Virology 93, 188-197, 1979b.

    Article  PubMed  CAS  Google Scholar 

  13. Martinez-Izquierdo J. and Hohn T., Proc Natl Acad Sci USA 84, 1824-1828, 1987.

    Article  PubMed  CAS  Google Scholar 

  14. Giband M., Mesnard J.M., and Lebeurier G., EMBO J 5, 2433- 2438, 1986.

    PubMed  CAS  Google Scholar 

  15. Mougeot J.L., Guidasci T., Wurch T., Lebeurier G., and Mesnard J.M., Proc Natl Acad Sci USA 90, 1470-1473, 1993.

    Article  PubMed  CAS  Google Scholar 

  16. Ménissier J., Laquel P., Lebeurier G., and Hirth L., Nucl Acids Res 12, 8769-8778, 1984.

    PubMed  Google Scholar 

  17. Covey S.N., Nucl Acids Res 14, 623-633, 1986.

    PubMed  CAS  Google Scholar 

  18. Fütterer J. and Hohn T., Trends Biochem Sci 12, 92-95, 1987.

    Article  Google Scholar 

  19. Hunter E., Sem Virol 5, 71-83, 1994.

    Article  CAS  Google Scholar 

  20. Taylor F.M. and Martindale D.W., Nucl Acids Res 21, 4601- 4614, 1993.

    Google Scholar 

  21. Scholthof H.B., Wu F.C., Kieman J.M., and Shepherd R.J., J Gen Virol 74, 775-780, 1993.

    PubMed  CAS  Google Scholar 

  22. Studier F.W. and Moffatt B.A., J Mol Biol 189, 113-130, 1986.

    Article  PubMed  CAS  Google Scholar 

  23. Gallina A., Bonelli F., Zentilin L., Rindi G., Muttini M., and Milanesi G., J Virol 63, 4645-4652, 1989.

    PubMed  CAS  Google Scholar 

  24. Bensadoun A. and Weinstein D., Anal Biochem 70, 241-250, 1976.

    Article  PubMed  CAS  Google Scholar 

  25. McEwen C.R., Analytical Biochem 20, 114-149, 1967.

    Article  CAS  Google Scholar 

  26. Hatton T., Zhou S., and Standring D.N., J Virol 66, 5232-5241, 1992.

    PubMed  CAS  Google Scholar 

  27. Sambrook J., Fritsch E.F., and Maniatis T., ``Molecular cloning: a laboratory manual'' Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 1989.

    Google Scholar 

  28. De Tapia M., Himmelbach A., and Hohn T., EMBO J 12, 3305- 3314, 1993.

    PubMed  CAS  Google Scholar 

  29. Hu G., Strydom D.J., Fett J.W., Riordan J.F., and Vallee B.L., Proc Natl Acad Sci USA 90, 1217-1221, 1993.

    Article  PubMed  CAS  Google Scholar 

  30. Daubert S., Richins R., Shepherd R.J., and Gardner R.C., Virology 122, 444-449, 1982.

    Article  CAS  PubMed  Google Scholar 

  31. Fütterer J., Gordon K., Pfeiffer P., and Hohn T., Gene 67, 141- 145, 1988.

    Article  PubMed  Google Scholar 

  32. Gordon K., Pfeiffer P., Fütterer J., and Hohn T., EMBO J 7, 309-317, 1988.

    PubMed  CAS  Google Scholar 

  33. Hahn P. and Shepherd R.J., Virology 116, 480-488, 1982.

    Article  CAS  PubMed  Google Scholar 

  34. Du Plessis D.H. and Smith P., Virology 109, 403-408, 1981.

    Article  CAS  PubMed  Google Scholar 

  35. Thompson S.R. and Melcher U., J Gen Virol 74, 1141-1148, 1993.

    Article  PubMed  CAS  Google Scholar 

  36. Freire R., Salas M., and Hermoso J.M., EMBO J 13, 4353- 4360, 1994.

    PubMed  CAS  Google Scholar 

  37. Crowther R.A., Kiselev N.A., Böttcher B., Berriman J.A., Borisova G.P., Ose V., and Pumpens P., Cell 77, 943-950, 1994.

    Article  PubMed  CAS  Google Scholar 

  38. Hwang D.J., Roberts I.M., and Wilson T.M.A., Proc Natl Acad Sci USA 91, 9067-9071, 1994.

    Article  PubMed  CAS  Google Scholar 

  39. Sastri M., Kekuda R., Gopinath K., Kumar C.T.R., Jagath J.R., and Savithri H.S., J Mol Biol 272, 541-552, 1997.

    Article  PubMed  CAS  Google Scholar 

  40. Campbell S. and Vogt V.M., J Virol 69(10), 6487-6497, 1995.

    PubMed  CAS  Google Scholar 

  41. Delchambre M., Gheysen D., Thines D., Thiriart C., Jacobs E., Verdin E., Horth M., Burny A., and Bex F., EMBO J 8, 2653- 2660, 1989.

    PubMed  CAS  Google Scholar 

  42. Klikova M., Rhee S.S., Hunter E., and Ruml T., J Virol 69(2), 1093-1098, 1995.

    PubMed  CAS  Google Scholar 

  43. Ehrlich L.S., Agresta B.E., and Carter C.A., J Virol 88, 4874- 4883, 1992.

    Google Scholar 

  44. Kräusslich H., Fäcke M., Heuser A., Konvalinka J., and Zentgraff H., J Virol 69, 3407-3419, 1995.

    PubMed  Google Scholar 

  45. Rossmann M.G. and Johnson J.E., Annu Rev Biochem 58, 533- 573, 1989.

    Article  PubMed  CAS  Google Scholar 

  46. Wills J.W. and Craven R.C., AIDS 5, 639-654, 1991.

    PubMed  CAS  Google Scholar 

  47. Scaglioni P.P., Melegari M., and Wands J.R., Virology 205, 112-120, 1994.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Friedrich-Miescher Institute, P.O. Box 2543, CH-4002, Basel, Switzerland

    Yvan Chapdelaine & Thomas Hohn

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  1. Yvan Chapdelaine
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  2. Thomas Hohn
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Chapdelaine, Y., Hohn, T. The Cauliflower Mosaic Virus Capsid Protein: Assembly and Nucleic Acid Binding In Vitro. Virus Genes 17, 139–150 (1998). https://doi.org/10.1023/A:1008064623335

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