Skip to main content

Oleic acid is the main fatty acid related with carotenogenesis in Dunaliella salina

Abstract

The variation of the fatty acid profile and the carotene content of Dunaliella salina in response to irradiance (80, 128, 640, 1000, 1500 μmol photon m−2 s−1) and nitrogen starvation were analysed. The highest fatty acid content per cell and the least polyunsaturated fatty acid percentage were exhibited under 1500 μmol photon m−2 s−1. Furthermore, the oleic acid (18:1) content maintained a positive and significant correlation with the carotene content per cell and with the irradiance. The composition of the carotene globules in Dunaliella salina may be the main determinant of this correlation.

This is a preview of subscription content, access via your institution.

References

  1. Al-Hasan RH, Ghannoum MA, Sallal A-K, Abu-Eteen KH, Radwan SS (1987) Correlative changes of growth, pigmentation and lipid composition of Dunaliella salina in response to halostress. J. gen. Microbiol. 133: 2607–2617.

    CAS  Google Scholar 

  2. Ben-Amotz A, Avron M (1983) On the factors which determine massive -carotene accumulation in the halotolerant alga Dunaliella bardawil. Plant Physiol. 72: 593–597.

    PubMed  CAS  Article  Google Scholar 

  3. Ben-Amotz A, Katz A, Avron M (1982) Accumulation of carotene in halotolerant algae: purification and characterisation of β-carotene globules from Dunaliella salina (Chlorophyceae). J. Phycol. 18: 529–537.

    CAS  Article  Google Scholar 

  4. Ben-Amotz A, Shaish A, Mordhay A (1989) Mode of action of the massively accumulated β-carotene of Dunaliella bardawil in protecting the alga against damage by excess irradiation. Plant Physiol. 91: 1040–1043.

    PubMed  CAS  Google Scholar 

  5. Berner T, Dubinsky A, Wyman K, Falkowski PG (1989) Photoadaptation and the ‘package’ effect in Dunaliella tertiolecta (Chlorophyceae). J. Phycol. 25: 70–78.

    CAS  Article  Google Scholar 

  6. Borowitzka LJ, Borowitzka MA, Moulton TP (1984) The mass culture of Dunaliella salina for fine chemicals: From laboratory to pilot plant. Hydrobiologia 116/117: 115–125.

    Article  Google Scholar 

  7. Cho SH, Thompson GA (1986) Properties of a fatty acid hydrolase preferentially attacking monogalactosyldiacylglycerols in Dunaliella salina chloroplasts. Biochim. Biophys. Acta 878: 353–359.

    CAS  Google Scholar 

  8. Fried A, Tietz A, Ben-Amotz A, Eichenberger W (1982) Lipid composition of the halotolerant alga, Dunaliella bardawil. Biochim. Biophys. Acta 713: 419–426.

    CAS  Google Scholar 

  9. Goes JI, Handa N, Taguchi S, Hama T (1994) Effect of UV-B radiation on the fatty acid composition of the marine phytoplankter Tetraselmis sp.: relationship to cellular pigments. Mar. Ecol. Progr. Ser. 114: 259–274.

    CAS  Google Scholar 

  10. Gómez-Pinchetti JL, Ramazanov ZM, Fontes AG, García Reina G (1992) Photosynthetic characteristics of Dunaliella salina (Chlorophyceae, Dunaliella) in relation to β-carotene content. J. Appl. Phycol. 4: 11–15.

    Article  Google Scholar 

  11. Jiménez del Río M, Ramazanov ZM, García-Reina G (1994) Dark induction of nitrate reductase in the halophilic alga Dunaliella salina. Planta 192: 40–45.

    Google Scholar 

  12. Katz A, Jiménez C, Pick U (1995) Isolation and characterisation of a protein associated with carotene globules in the alga Dunaliella bardawil. Plant Physiol. 108: 1657–1664.

    PubMed  CAS  Google Scholar 

  13. Kivic PA, Vesk M (1972) Structure and function in the euglenoid eyespot apparatus: the fine structure, and response to environmental changes. Planta 105: 1–14.

    Article  Google Scholar 

  14. Mendoza H, Jiménez del Río M, García Reina G, Ramazanov ZM (1996) Low temperature induced β-carotene and fatty acid synthesis, and ultrastructural reorganization of the chloroplast in Dunaliella salina. Eur. J. Phycol. 31: 329–331.

    Google Scholar 

  15. Petkov GD, Klyachko-Gruvich GD, Furnadjieva ST, Pronina NA, Ramazanov ZM (1990) Genotypic differences and phenotypic changes of lipid and fatty acid composition in strains of Dunaliella salina. Sov. Plant Physiol. 3: 268–272.

    Google Scholar 

  16. Roessler PG (1990) Environmental control of glycerolipid metabolism in microalgae: commercial implications and future research directions. J. Phycol. 26: 393–399.

    CAS  Article  Google Scholar 

  17. Semenenko VE, Abdullaev AA (1980) Parametric control of β-carotene biosynthesis in Dunaliella salina cells under conditions of intensive cultivation. Sov. Plant. Physiol. 27: 22–30.

    Google Scholar 

  18. Sukenik A, Carmeli Y, Berner T (1989) Regulation of fatty acid composition by irradiance level in the eustigmatophyte Nannochloropsis sp. J. Phycol. 24: 445–452.

    Google Scholar 

  19. Thompson PA, Harrison PJ, Whyte JN (1990) Influence of irradiance on the fatty acid composition of phytoplankton. J. Phycol. 26: 278–288.

    CAS  Article  Google Scholar 

  20. Vechel B, Eichenberger W, Ruppel HG (1992) Lipid bodies in Eremosphaera viridis De Bary (Chlorophyceae). Plant Cell Physiol. 33: 41–48.

    Google Scholar 

  21. Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J. Plant Physiol. 144: 307–313.

    CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to H. Mendoza.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mendoza, H., Martel, A., Jiménez del Río, M. et al. Oleic acid is the main fatty acid related with carotenogenesis in Dunaliella salina. Journal of Applied Phycology 11, 15–19 (1999). https://doi.org/10.1023/A:1008014332067

Download citation

  • carotene
  • Dunaliella salina
  • fattyacid
  • irradiance
  • nitrogen starvation
  • oleic acid (18:1)