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Nitrogen availability influences the biochemical composition and photosynthesis of tank-cultivated Ulva rigida (Chlorophyta)

Journal of Applied Phycology volume 10, pages 383–389 (1998)Cite this article

Abstract

Physiological and biochemical changes in relation to inorganic nitrogen availability were studied for tank-cultivated Ulva rigida grown under nitrogen- enriched and nitrogen-depleted seawater. U. rigida was initially cultivated in nitrogen-enriched seawater (daily concentrations of NH4+ and NO3- + NO2- ranged between 0.5–1.7 and 0.06–0.15 mg L-1, respectively), then transferred to nitrogen-depleted seawater where photosynthetic capacity decreased to zero after 23 d. At the time (14 d) when photosynthetic rates were lower than 2.0 μmol O2 g-1 FW min-1 and strong bleaching had occurred, some algae were returned to the initial nitrogen-enriched seawater to study recovery from N-limited growth. Data on biochemical composition (chlorophylls, ash, caloric content, fatty acids and dietary fibres) and colouration varied significantly depending on the nitrogen conditions. C:N ratios correlated significantly with biochemical parameters. Fatty acid (FA) synthesis continued during the N-starvation period; saturated and mono-unsaturated FA increased to a maximun of 72.2%, while poly-unsaturated fatty acids (PUFA) decreased to 27.7%. During the N-enriched recovery period, the reverse was found. C:N ratios above 10 correlated with carbohydrate synthesis as shown by the dietary fibre level. Under nitrogen enriched conditions, C:N ratios decreased along with a decrease in fibre level. Under controlled conditions, nitrogen represents a major influence on the development of intensive tank cultivation of Ulva rigida, not only by affecting parameters closely related to nitrogen metabolism but also some clearly influenced by carbon uptake.

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References

  • Bar E, Rise M, Vishkautsan M, Arad SM (1995) Pigment and structural changes in Chlorella zofingiensisupon light and nitrogen stress. J. Plant Physiol. 146: 527-534.

    CAS  Google Scholar 

  • Betancort JR (1997) Determinación de aminoácidos por HPLC. Estudio cualitativo y cuantitativo de aminoácidos libres en algas y su variación por condiciones de cultivo. Ph.D. Thesis. Univ. de Las Palmas de G.C. 135 pp.

  • Björnsäter BR, Wheeler PA (1990) Effect of nitrogen and phosphorus supply on growth and tissue composition of Ulva fenestrataand Enteromorpha intestinalis(Ulvales, Chlorophyta). J. Phycol. 26: 603-611.

    Article  Google Scholar 

  • Chapman AR, Craigie JS (1977) Seasonal growth in Laminaria longicruris: relations with dissolved inorganic nutrients and internal reserves of nitrogen. Mar. Biol. 40: 197-205.

    Article  CAS  Google Scholar 

  • Chen F, Johns MR (1991) Effect of C/N ratio and aeration on the fatty acid composition of heterotrophic Chlorella sorokiniana. J. appl. Phycol. 3: 203-209.

    Article  CAS  Google Scholar 

  • Cohen Z, Vonshak A, Richmond A (1987) Fatty acid composition of Spirulinastrains grown under various environmental conditions. Phytochemistry 26: 2255-2258.

    Article  CAS  Google Scholar 

  • Coutinho R, Zingmark R (1993) Interactions of light and nitrogen on photosynthesis and growth of the marine macroalga Ulva curvata(Kützing) De Toni. J. exp. mar. Biol. Ecol. 167: 11-19.

    Article  Google Scholar 

  • Darcy-Vrillon B (1993) Nutritional aspects of the developing use of marine macroalgae for the human food industry. Int. J. Food Sci. Nutr. 44: S23-S35.

    Google Scholar 

  • Davies SJ, Brown MT, Camilleri M (1997) Preliminary assessment of the seaweed Porphyra purpureain artificial diets for thick-lipped grey mullet (Chelon labrosus). Aquaculture 152: 249-258.

    Article  Google Scholar 

  • D'Elia CF, DeBoer JA (1978) Nutritional studies of two red algae. II. Kinetics of of ammonium and nitrate uptake. J. Phycol. 14: 266-272.

    Google Scholar 

  • Duke CS, Lapointe BE, Ramus J (1986) Effects of light on growth, RuBPCase activity and chemical composition of Ulvaspecies (Chlorophyta). J. Phycol. 22: 362-370.

    CAS  Google Scholar 

  • Floreto EAT, Teshima S, Ishikawa M (1996) Effects of nitrogen and phosphorus on the growth and fatty acid composition of Ulva pertusaKjellman (Chlorophyta). Bot. mar. 39: 69-74.

    Article  CAS  Google Scholar 

  • García-Ferris C, de los Ríos A, Ascaso C, Moreno J (1996) Correlated biochemical and ultrastructural changes in nitrogen-starved Euglena gracilis. J. Phycol. 32: 953-963.

    Article  Google Scholar 

  • Hanisak MD (1979) Nitrogen limitation of Codium fragilessp. tomentosoidesas determined by tissue analysis. Mar. Biol. 50: 333-337.

    Article  CAS  Google Scholar 

  • Hanisak MD (1983) The nitrogen relationships of marine macroalgae. In: Carpenter EJ, Capone DG (eds), Nitrogen in the Marine Environment, Academic Press, New York: 699-730.

    Google Scholar 

  • Himmelman JH, Carefoot TH (1975) Seasonal changes in calorific value of three Pacific coast seaweeds, and their significance to some marine invertebrate herbivores. J. exp. mar. Biol. Ecol. 18: 139-151.

    Article  Google Scholar 

  • Jeffrey SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophyll a, b, c1, c2in higher plants, algae and natural phytoplankton. Biochem. Physiol. Pflanz. 167: 191-194.

    CAS  Google Scholar 

  • Jiménez del Rio M, Ramazanov Z, García Reina G (1995) Effect of nitrogen supply on photosynthesis and carbonic anhydrase activity in the green seaweed Ulva rigida(Chlorophyta). Mar. Biol. 123: 687-691.

    Article  Google Scholar 

  • Jiménez del Rio M, Ramazanov Z, García Reina G (1996) Ulva rigida(Ulvales: Chlorophyta) tank culture as biofilters for dissolved inorganic nitrogen from fishpond effluents. Hydrobiologia 326/327: 61-66.

    Article  Google Scholar 

  • Jones AB, Dennison WC, Stewart GR (1996) Macroalgal responses to nitrogen source and availability: amino acid metabolic pro-filing as a bioindicator using Gracilaria edulis(Rhodophyta). J. Phycol. 32: 757-766.

    Article  CAS  Google Scholar 

  • Lahaye M (1991) Marine algae as sources of fibres: determination of soluble and insoluble dietary fibre contents in some sea vegetables. J. Sci. Food Agric. 54: 587-594.

    CAS  Google Scholar 

  • Lahaye M, Gómez-Pinchetti JL, Jiménez del Río M, García-Reina G (1995) Natural decoloration, composition and increase in dietary fibre content of an edible marine algae, Ulva rigida(Chlorophyta), grown under different nitrogen conditions. J. Sci. Food Agric. 68: 99-104.

    CAS  Google Scholar 

  • Lapointe BE, Ryther JH (1979) The effects of nitrogen and seawater flow rate on the growth and biochemical composition of Gracilaria foliiferavar. angustissimain mass outdoor cultures. Bot. mar. 22: 529-537.

    CAS  Google Scholar 

  • Lobban CS, Harrison PJ (1994) Seaweed Ecology and Physiology. Cambridge University Press, New York, 366 pp.

    Google Scholar 

  • McGlathery KJ, Pedersen MF, Borum J (1996) Changes in intracellular nitrogen pools and feedback controls on nitrogen uptake in Chaetomorpha linum(Chlorophyta). J. Phycol. 32: 393-401.

    Article  CAS  Google Scholar 

  • Neori A, Cohen I, Gordin H (1991) Ulva lactucabiofilters for marine fishpond effluents. II. Growth rate, yield and C:N ratio. Bot. mar. 34: 483-489.

    Article  Google Scholar 

  • Pohl P, Zurheide F (1979) Fatty acids and lipids of marine algae and the control of their biosynthesis by environmental factors. In: Hoppe HA, Levring T, Tanaka Y (eds), Marine Algae in Pharmaceutical Science, Walter de Gruyter, Berlin: 473–523.

    Google Scholar 

  • Smit AJ, Robertson BL, du Preez DR (1997) Influence of ammonium-N pulse concentrations and frequency, tank condition and nitrogen starvation on growth rate and biochemical composition of Gracilaria gracilis. J. appl. Phycol. 8: 473-481.

    Article  Google Scholar 

  • Turpin DH (1991) Effects of inorganic N availability on algal photosynthesis and carbon metabolism. J. Phycol. 27: 14-20.

    Article  CAS  Google Scholar 

  • Vergara JJ, Niell FX, Torres M (1993) Culture of Gelidium sesquipedale(Clem.) Born. etThur. in a chemostat system. Biomass production and metabolic responses affected by N flow. J. appl. Phycol. 5: 405-415.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Instituto de Algología Aplicada, Universidad de Las Palmas de G.C., Muelle de Taliarte s/n, 35214, Telde, Las Palmas, Canary Islands, Spain

    Juan Luis Gómez Pinchetti, Elena del Campo Fernández, Paula Moreno Díez & Guillermo García Reina

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  1. Juan Luis Gómez Pinchetti
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  2. Elena del Campo Fernández
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  3. Paula Moreno Díez
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  4. Guillermo García Reina
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Gómez Pinchetti, J.L., del Campo Fernández, E., Moreno Díez, P. et al. Nitrogen availability influences the biochemical composition and photosynthesis of tank-cultivated Ulva rigida (Chlorophyta). Journal of Applied Phycology 10, 383–389 (1998). https://doi.org/10.1023/A:1008008912991

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  • DOI: https://doi.org/10.1023/A:1008008912991

  • ammonium
  • C:N ratio
  • tank culture
  • dietary fibre
  • fatty acids
  • nitrogen
  • photosynthesis
  • Ulva rigida