Growth of a dissociated, filamentous stage of Codium species in laboratory culture
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Unialgal cultures were established from isolated utricles for all five Codium species occurring in the British Isles and growth studies performed under different conditions of temperature (10-30 °C), photon irradiance (0-175 µmol m-2 s-1) and salinity (0-100% seawater growth medium). The species studied were C. fragile ssp. tomentosoides, C. fragile ssp. atlanticum, C. adhaerens, C. bursa, C. tomentosum and C. vermilara. All cultures developed into growth forms comprised of branched, dissociated, coenocytic filaments. All grew rapidly in culture, although the conditions for optimum growth sometimes differed for the individual entities. The development of typical Codium plants, with utricles, similar to the parental material, occurred only occasionally. The rapid growth of the dissociated, filamentous forms in laboratory culture led to the provision of sufficient quantities of plant material to enable detailed studies to be made of the lectins present in several of the species.
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