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Deletion Analysis of Both the Long Terminal Repeat and the Internal Promoters of the Human Foamy Virus

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Abstract

Deletion analyses of the long terminal repeat (LTR) and internal promoters (IP) of human foamy virus (HFV) showed that a negative acting element resides in the U5 region of the 5′ LTR reducing reporter gene expression tenfold. The basal activity of the IP was higher than that obtained with LTR promoter constructs and strongly elevated in permissive BHK-21 cells whereas semi-permissive COS-7 cells showed low basal activity. Since the basal activity of the IP is critical for initiating HFV gene expression by providing Bel 1 transactivator early after infection, the basal activity of the IP may be the crucial factor that contributes to whether cells are permissive for HFV infection or not. Deletion mutagenesis allowed to define the minimal IP region. A region strongly transactivated by Bel 1 extends from −136 to +58 relative to the cap site of the IP. The major Bel 1 response element of the IP required for transactivation is located upstream of the cap site between −136 and −88 relative to the internal cap site. A DNA fragment reported to be protected by recombinant Bel 1 was deleted with marginal reduction of Bel 1 transactivation. HFV gene expression directed by the IP and LTR promoters is thus multiply regulated by positive and negative acting response elements in cis and their binding partners in trans.

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References

  1. Achong B.C., Mansell P.W., Epstein M.A., and Clifford P., J Natl Cancer Inst 46, 299–307, 1971.

    PubMed  CAS  Google Scholar 

  2. Löchelt M. and Flügel R.M., in Levy J.A. (ed.), The Retroviridae, Plenum Press. Vol 4, pp. 239–292, 1995.

  3. Muranyi W. and Flügel R.M., J Virol 65, 727–735, 1991.

    PubMed  CAS  Google Scholar 

  4. Löchelt M., Flügel R.M., and Aboud M., J Virol 68, 638–645, 1994.

    PubMed  Google Scholar 

  5. Yu. S.F., Baldwin D.N., Gwynn S.R., Yendapalli S., and Linial M.L., Science 271, 1579–1582, 1996.

    PubMed  CAS  Google Scholar 

  6. Bodem J., Löchelt M., Winkler I., Flower R.P., Delius H. and Flügel R.M., J Virol 70, 9024–9027, 1996.

    PubMed  CAS  Google Scholar 

  7. Löchelt M., Muranyi W., and Flügel, R.M., Proc Natl Acad Sci USA 90, 7317–7321, 1993.

    Article  PubMed  Google Scholar 

  8. Keller A., Partin K.M., Löchelt M., Bannert H., Flügel R.M. and Cullen B.R., J Virol 65, 2589–2594, 1991.

    PubMed  CAS  Google Scholar 

  9. Rethwilm A., Erlwein, O., Baunach, G., Maurer, B., and ter Meulen, V., Proc Natl Acad Sci USA 88, 941–945, 1991.

    Article  PubMed  CAS  Google Scholar 

  10. He F., Sun J.D., Garrett E.D., and Cullen, B.R., J Virol 67, 1896–1904, 1993.

    PubMed  CAS  Google Scholar 

  11. Lee C.W., Chang J., Lee K.J., Kim S., and Sung Y.C., J Virol 68, 2708–2719, 1994.

    PubMed  CAS  Google Scholar 

  12. Venkatesh L.L. and Chinnadurai G., J Virol 67, 3868–3876, 1993.

    PubMed  CAS  Google Scholar 

  13. Ventatesh L.K., Yang C., Theodorakis P.A., and Chinnadurai G., J Virol 67, 161–169, 1993.

    Google Scholar 

  14. Chang J., Lee K.J., Jang K.L., Lee E.K., Baek G.H., and Sung Y.C., J Virol 69, 801–808, 1995.

    PubMed  CAS  Google Scholar 

  15. Mergia A., Renshaw-Gegg L.W., Stout M.W., Renne R., and Herchenröder O., J Virol 67, 4598–4604, 1993.

    PubMed  CAS  Google Scholar 

  16. Löchelt M., Zentgraf H., and Flügel R.M., Virology 184, 43–54, 1991.

    PubMed  Google Scholar 

  17. Yu S.F. and Linial M.L., J Virol 67, 6618–6624, 1993.

    PubMed  CAS  Google Scholar 

  18. Baunach G., Maurer B., Hahn H., Kranz M., and Rethwilm, A., J Virol 67, 5411–5418, 1993.

    PubMed  CAS  Google Scholar 

  19. Löchelt M., Yu S.F., Linial M.L., and Flügel R.M., Virology 206, 601–610, 1995.

    Article  PubMed  Google Scholar 

  20. Keller A., Garrrett E.D., and Cullen B.R., J Virol 66, 3946–3949, 1992.

    PubMed  CAS  Google Scholar 

  21. Lee A.H., Lee K.J., Kim S., and Sung Y.C., J Virol 66, 3236–3240, 1992.

    PubMed  CAS  Google Scholar 

  22. Lee K.J., Lee A.H., and Sung Y.C., J Virol 67, 2317–2326, 1993.

    PubMed  CAS  Google Scholar 

  23. Mergia A., Pratt-Lowe E., Shaw K.E.S., Renshaw L.W., and Luciw P.A., J Virol 66, 251–257, 1992.

    PubMed  CAS  Google Scholar 

  24. Erlwein O. and Rethwilm A., Virology 196, 256–268, 1993.

    Article  PubMed  CAS  Google Scholar 

  25. Campbell M., Renshaw-Gegg L., Renne R., and Luciw P.A., J Virol 68, 4811–4820, 1994.

    PubMed  CAS  Google Scholar 

  26. Zhou J.X. and Luciw P.A., Proc Natl Acad Sci USA 93, 326–330, 1996.

    Article  Google Scholar 

  27. He F., Blair W.S., Fukushima J., and Cullen B.R., J Virol 70, 3902–3908, 1996.

    PubMed  CAS  Google Scholar 

  28. Boshart M., Klüppel M., Schmidt A., Schütz G., and Luckow B., Gene 110, 129–130, 1992.

    Article  PubMed  CAS  Google Scholar 

  29. Döffinger R., Pawlita M., and Sczakiel G., Nucl Acids Res 16, 11840, 1988.

    PubMed  Google Scholar 

  30. Gorman C.M., Moffat L.F., and Howard B.H., Mol Cell Biol 2, 1044–1051, 1982.

    PubMed  CAS  Google Scholar 

  31. Maniatis T., Sambrook J., and Fritsch E.F., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor University Press, Cold Spring Harbor, NY., 1989.

    Google Scholar 

  32. de Wet J.R., Wood K.V., DuLuca M., Helsinki D.R., and Subramani S., Mol Cell Biol 7, 725–737, 1987.

    PubMed  CAS  Google Scholar 

  33. Hoppe-Seyler F., Butz K., Rittmüller C., and von Knebel Doeberitz M., Nucl Acids Res 19, 5080, 1991.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Abteilung Retrovirale Genexpression, Forschungsschwerpunkt Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 242, 69009, Heidelberg, Germany

    Ping Yang, Motomi Zemba, Rolf M. Flügel & Martin Löchelt

  2. Dept. of Microbiology, Ben-Gurion University of the Negev, Beer Sheva, Israel

    Mordechai Aboud

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  1. Ping Yang
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  2. Motomi Zemba
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  3. Mordechai Aboud
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  4. Rolf M. Flügel
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  5. Martin Löchelt
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Yang, P., Zemba, M., Aboud, M. et al. Deletion Analysis of Both the Long Terminal Repeat and the Internal Promoters of the Human Foamy Virus. Virus Genes 15, 17–23 (1997). https://doi.org/10.1023/A:1007994527345

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