Skip to main content
SpringerLink
Log in

The Characterization of a Subgenomic RNA and In Vitro Translation Products of Oat Chlorotic Stunt Virus

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Oat chlorotic stunt virus (OCSV) is a 35 nm icosahedral plant virus comprising of a single capsid protein with a Mr of 48.2 kDa and a 4.1 kb single stranded, positive sense genomic RNA. Northern blot analysis detected a single 3′ terminal subgenomic RNA in extracts from infected plants, which was also found to be encapsidated. Virion RNA directs the synthesis of a 23 kDa polypeptide in a rabbit reticulocyte in vitro translation system. Primer extension analysis has been used to map the end of both the genomic and subgenomic RNA’s, and has shown the genomic size to be 4115 nucleotides in length. The results have enabled a model for the genome expression to be proposed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Boonham N., Henry C.M., and Wood K.R., J Gen Virol 76, 2025–2034, 1995.

    Article  CAS  PubMed  Google Scholar 

  2. Logemann J., Schell J., and Willmitzer L., Analytical Biochemistry 163, 16–30, 1987.

    Article  CAS  PubMed  Google Scholar 

  3. Brakke M. and Van Pelt N. Analytical Biochemistry 38, 56–64, 1970.

    Article  CAS  PubMed  Google Scholar 

  4. Sambrook J., Fritsh E.F., and Maniatis T., Molecular Cloning: a laboratory manual. Cold Spring Harbor Press, 1989.

  5. Matthews, R.E.F., Plant Virology. Academic Press, 1991.

  6. Lommel S.A., Kendall T.L., Xiong Z., and Nutter R.C., Virology 181, 382–385, 1991.

    Article  CAS  PubMed  Google Scholar 

  7. French R. and Ahlquist P., Journal of Virology 61, 1457–1465, 1987.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Lutke H.A., Chow K.C., Mickel F.S., Moss K.A., Kern H.F., and Scheele G.A., EMBO Journal 6, 43–48, 1991.

    Article  Google Scholar 

  9. Heaton L.A., Lee T.C., Wei N., and Morris T.J., Virology 183, 143–150, 1991.

    Article  CAS  PubMed  Google Scholar 

  10. Hacker D.L., Petty I.T.D., Wei N., and Morris T.J., Virology 186, 1–8, 1992.

    Article  CAS  PubMed  Google Scholar 

  11. Offei S.K., Coffin R.S., and Coutts R.H.A., J Gen Virol 76, 1493–1496, 1995.

    Article  CAS  PubMed  Google Scholar 

  12. Dalmay T., Rubino L., Burgyan J., and Russo M., Molecular Plant Microbe Interactions 5, 379–383, 1992.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Central Science Laboratory, Sand Hutton, York, YO4 1LZ, UK

    Neil Boonham & Christine M. Henry

  2. The School of Biological Sciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK

    K. Roger Wood

Authors
  1. Neil Boonham
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christine M. Henry
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Roger Wood
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boonham, N., Henry, C.M. & Wood, K.R. The Characterization of a Subgenomic RNA and In Vitro Translation Products of Oat Chlorotic Stunt Virus. Virus Genes 16, 141–145 (1998). https://doi.org/10.1023/A:1007985404933

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1007985404933

Search

Navigation