Abstract
An ecdysteroid UDP-glucosyltransferase (egt) gene was identified from the single (S) nucleocapsid nucleopolyhedrovirus of Heliothis armigera (HearNPV). In baculovirus-infected insects the viral enzyme (EGT) plays a pivotal role in abrogating the insect molting process. The open reading frame of the egt gene is 1545 nucleotides long, encoding a putative protein of 515 amino acids with a M r of 59.1. The 5′-noncoding region contains a putative early (CAGT) and late (TAAG) motif for transcription initiation, a transcription enhancer sequence (CGTCGC) and two TATA boxes. A putative polyA signal, AATAAA, was found downstream of the translation stop codon. A putative signal peptide of 21 residues was present at the N-terminus of the EGT. The HearNPV egt gene has a high degree of nucleotide and amino acid sequence homology to the egt genes of Buzura suppressaria SNPV and Spodoptera exigua MNPV. The HearNPV EGT shares ten conserved motifs with other EGTs. A phylogenetic tree of twelve baculovirus EGTs was constructed by using maximum parsimony analysis, suggesting that SNPVs do not form a separate clade within the baculovirus family.
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Murphy F.A., Fauquet C.M., Bishop D.H.L., Ghabrial S.A., Jarvis A.W., Martelli G.P., Mayo M.A., and Summers M.D. (eds.). Virus Taxonomy-The Classification and Nomenclature of Viruses: Sixth Report of the International Committee on Taxonomy of Viruses. Springer-Verlag, New York, 1995.
O'Reilly D.R., Insect Biochem Mol Biol 25, 541-550, 1995.
O'Reilly D.R. and Miller L.K., Science 245, 1110-1112, 1989.
O'Reilly D.R. and Miller L.K., Bio/Technol 9, 1086-1089, 1991.
Flipsen J.T.M., Mans R.M.W., Kleefsman A.W.F., Knebel-Mürsdorf D., and Vlak J.M., J Virol 69, 4529-4532, 1995.
Sun X. and Zhang G., Virol Sin 9, 309-318, 1994.
Zhang G., The IPM Practitioner 11, 13, 1989.
Zhang G., Resources and Environment in the Yangtze Valley 3, 1-6, 1994.
Bonning B.C. and Hammock B.D., Ann Rev Entomol 41, 191-210, 1996.
Hu Z.H. and Vlak J.M., Virol Sin 12, 14-25, 1997.
Chen X., Vlak J.M., and Hu Z.H., Virol Sin, 1997.
Chen X., Li M., Hu Z.H., and Vlak J.M., personal communication.
Cowan P., Bulach D., Goodge K., Robertson A., and Tribe D.E., J Gen Virol 75, 3211-3218, 1994.
Hu Z.H., Broer R., Westerlaken J., Martens J.W.M., Yin F., Jehle J.A., Wang L.M., and Vlak J.M., Virus Res 47, 91-97, 1997.
Southern E.M., J Mol Biol 98, 503-517, 1995.
Sambrook J., Fritsch E.F., and Maniatis T., Molecular Cloning. A laboratory guide. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989.
Devereux J., Heaberli P., and Smithies O., Nucl Acid Res 12, 387-395, 1984.
Swofford D.L. PAUP: Phylogenetic analysis using parsimony. Version 3.1. Computer program distributed by the Illinois Natural History Survey, Champaign, IL, 1993.
Guarino L.A. and Smith M., J Virol 66, 3733-3739, 1992.
O'Reilly D.R. and Miller L.K., J Virol 64, 1321-1328, 1990.
Riegel C.I., Lanner-Herrera C., and Slavicek J.M., J Gen Virol 75, 829-838, 1994.
Barrett J.W., Krell P.J., and Arif B.M., J Gen Virol 76, 2447-2456, 1995.
Clarke E.E., Tristem M., Cory J.S., and O'Reilly D.R., J Gen Virol 77, 2865-2871, 1996.
Zanotto P.M. de A., Kessing B.D., and Maruniak J.E., J Invertebr Pathol 62, 147-164, 1993.
Faktor O., Toister-Achituv M., and Kamensky B., Virus Genes 11, 47-52, 1995.
Ahrens C.H., Russell R.L.Q., Funk C.J., Evans J.T., Harwood S.H., and Rohrmann G.F., Virology 229, 318-399, 1997.
Smith I.R.L. and Goodale C., J Gen Virol 79, 1997.
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Chen, X., Hu, Z., Jehle, J.A. et al. Analysis of the Ecdysteroid UDP-Glucosyltransferase Gene of Heliothis armigera Single-Nucleocapsid Baculovirus. Virus Genes 15, 219–225 (1997). https://doi.org/10.1023/A:1007976420021
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DOI: https://doi.org/10.1023/A:1007976420021