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Development of a versatile computer integrated control system for bioprocess controls

Cytotechnology volume 26, pages 227–236 (1998)Cite this article

Abstract

A general approach is described for the implementation of a networked multi-unit computer integrated control system. The use of data acquisition hardware and graphical programming tools alleviates tedious programming and maintains potency and flexibility. One application of the control system, the control of a mammalian cell perfusion culture based on a key nutrient glucose concentration, was demonstrated. The control system offers customized user interface for all process control parameters and allows the flexibility for continued improvement and implementation of new tailored functions. The temperature, pH, dissolved oxygen and glucose level were accurately controlled.

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References

  • Buntemeyer H, Marzahl R & Lehmann J (1994) A direct computer control concept for mammalian cell fermentation processes. Cytotechnology 15(1-3): 271-279.

    PubMed  CAS  Article  Google Scholar 

  • Diaz C, Dieu P, Feuillerat C, Lelong P & Salome m (1995) Adaptive predictive control of dissolved oxygen concentration in a laboratory-scale bioreactor. J. Biotechnol. 43(1): 21-32.

    PubMed  CAS  Article  Google Scholar 

  • Karim MN & Rivera SL (1992) Artificial neural networks in bioprocess state estimation. Adv. Biochem. Eng. Biotechnol. 46: 1-33.

    PubMed  CAS  Google Scholar 

  • Knorre WA, Deckwer WD, Korz D, Pohl HD, Riesenberg D, Ross A, Sanders E & Schulz V (1991) High cell density fermentation of recombinant Escherichia coliwith computer-controlled optimal growth rate. Ann. N. Y. Acad. Sci. 646: 300-306.

    PubMed  CAS  Google Scholar 

  • Konstantinov KB (1996) Monitoring and control of the physiological state of cell cultures. Biotech. Bioeng. 52(2): 271-289.

    CAS  Article  Google Scholar 

  • Konstantinov KB, Tsai Y, Moles D & Matanguihan R (1996) Control of long-term perfusion Chinese hamster ovary cell culture by glucose auxostat. Biotechnol. Prog. 12(1): 100-109.

    PubMed  CAS  Article  Google Scholar 

  • Kyung YS, Peshwa MV, Gryte DM & Hu WS (1994) High density culture of mammalian cells with dynamic perfusion based on on-line oxygen uptake rate measurements. Cytotechnology 14(3): 183-190.

    PubMed  CAS  Article  Google Scholar 

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Affiliations

  1. Human Genome Sciences Inc., 9410 Key West Ave., Rockville, MD, 20850, U.S.A

    Deyu Kong, Reiner Gentz & Junli Zhang

Authors
  1. Deyu Kong
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  2. Reiner Gentz
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  3. Junli Zhang
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Kong, D., Gentz, R. & Zhang, J. Development of a versatile computer integrated control system for bioprocess controls. Cytotechnology 26, 227–236 (1998). https://doi.org/10.1023/A:1007948313304

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  • DOI: https://doi.org/10.1023/A:1007948313304

  • bioreactor
  • computer control
  • data acquisition
  • glucose control