Abstract
The complete nucleotide sequences of neuraminidase (NA) of two swine influenza viruses (H1N1) are presented. A/Sw/Quebec/5393/91 (SwQc91) virus, associated with the chronic respiratory disease and A/Sw/Quebec/192/81 (SwQc81) virus, associated with the acute respiratory disease, were used. The deduced amino acid sequences of NA of SwQc91 and SwQc81 viruses showed a high degree (>95%) of similarity. The NA gene of both viruses was a single open reading frame of 1459 nucleotides coding for 469 aa with a 5′ noncoding region of 21 nucleotides and a 3′ noncoding region of 28 nucleotides. The comparison of two sequences showed that there were 23 differences recorded for SwQc91 strain, of which 5, 6, and 12 differences were recorded in the hydrophobic, stalk and head regions, respectively. A potential antigenic determinant changed from Ala to Thr at position 453 and there was a new potential glycosylation site present at position 88 for SwQc91 strain whereas it was absent at position 50 when compared with SwQc81 strain. Estimates of genetic distance and phylogenic tree analysis showed that SwQc91 and SwQc81 viruses were closely related with each other and with the American strain, A/Sw/Wisconsin/4754/94. However, the swine viruses represented a distinct group that was considerably divergent from the group of human viruses.
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Chambers T.M., Hinshaw V.S., Kawaoka Y., Easterday B.C., and Webster R.J., Arch Virol 116, 261–265, 1991.
Easterday B.C., in Leman A.D., Straw B., Glock R.D., Mengeling W.L., Penny R.H.C., and Scholl E. (ed.). Diseases of swine, 6th ed. Iowa State University Press, Ames, 1986, pp. 244–255.
Schultz U., Fitch W., Ludwig M.S., Mandler J., and Scholtissek C., Virology 183, 61–71, 1991.
Morin M., Girard C., and Elazhary Y., Can Vet J 31, 837–839, 1990.
Girard C., Morin M., and Elazhary Y., Vet Rec 130, 206–207, 1992.
Rekik M.R., Arora D.J.S., and Dea S., J Clin Microbiol 32, 515–518, 1994.
Arora D.J.S., Tremblay S., Bourgault P.R., and Boileau S., Anal Biochem 144, 189–192, 1985.
Sambrook J., Fritsch E.F., and Maniatis T., Molecular Cloning: A laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York, 1989.
Schreier E., Petzold D.R., Michel S., and Dittmann S., Arch Virol 99, 271–276, 1988.
Concannon P., Kwolek C.J., and Salser W.A., J Virol 50, 654– 656, 1984.
Nei M., Molecular evolutionary genetics. Colombia University Press, New York, 1987, pp. 293–298.
Luoh S.M., McGregor M.W., and Hinshaw V.S., J Virol 66, 1066–1073, 1992.
Arora D.J.S., N'Diaye M., and Dea S., Arch Virol 142, 407– 412, 1997.
Bikour M.H., Frost E., Deslandes S., Talbot B., and Elazhary Y., J Gen Virol 76, 2539–2547, 1995.
Olsen C.W., McGregor M.W., Cooley A.J., Schantz B., Hotze B., and Hinshaw V.S., Am J Vet Res 54, 1630–1636, 1993.
Sheerar M.G., Easterday C., and Hinshaw V.S., J Gen Virol 70, 3297–3303, 1989.
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Henrichon, M., Tijssen, P., Dea, S. et al. Complete Sequences of the Neuraminidase Genes of Swine Influenza Viruses (H1N1) Associated with the Respiratory Disease in Pigs. Virus Genes 14, 251–254 (1997). https://doi.org/10.1023/A:1007944228967
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DOI: https://doi.org/10.1023/A:1007944228967