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Cytotechnology

, Volume 26, Issue 1, pp 23–27 | Cite as

Stimulation of cell growth in the U-937 human myeloid cell line by honey royal jelly protein

  • Kazuhiko Watanabe
  • Hiroshi Shinmoto
  • Masuko Kobori
  • Tojiro Tsushida
  • Kazuki Shinohara
  • Jun Kanaeda
  • Masami Yonekura
Article

Abstract

Royal jelly was fractionated by ion-exchange chromatography and a protein (DIII protein) that had growth stimulating activity to the U-937 human myeloid cell line was obtained. The molecular weight of the DIII protein was 58 kDa on SDS-PAGE. The growth stimulating activity of the DIII protein was shown to be relatively heat and pH stable.

cell differentiation growth factor royal jelly U-937 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Kazuhiko Watanabe
    • 1
  • Hiroshi Shinmoto
    • 2
  • Masuko Kobori
    • 2
  • Tojiro Tsushida
    • 2
  • Kazuki Shinohara
    • 3
  • Jun Kanaeda
    • 4
  • Masami Yonekura
    • 5
  1. 1.Ito-en Co. Ltd.Sagara-cho, ShizuokaJapan
  2. 2.Ministry of Agriculture, Forestry and FisheriesNational Food Research InstituteTsukuba, IbarakiJapan
  3. 3.Fisheries AgencyNational Research Institute of Fisheries ScienceKanagawaJapan
  4. 4.API Co. Ltd.Research InstituteGifuJapan
  5. 5.School of AgricultureIbaraki UniversityIbarakiJapan

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