Abstract
Turkey meningoencephalitis virus (TMEV) causes paralysis and mortality in turkeys. Because the classical diagnostic methods are complicated, we developed the RT-PCR as a new molecular diagnostic method. Since the nucleic acid sequence of TMEV is unknown, the first step in developing the RT-PCR relied on conserved sequences of viruses belonging to the Flaviviridae family, in which TMEV has been classified serologically. Using primers from the NS5 gene, three amplification products of TMEV RNA were obtained (125 bp, 181 bp and 800 bp). Their sequences were homologous to one another and to the NS5 gene of other flaviviruses.
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References
Altshul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J., J Mol Biol 215, 403–410, 1990.
Barnard B.J.H., Buys S.B., Du Prez J.H., Greyling S.P., and Vender H.J., J Vet Res 47, 89–94, 1980.
Braverman Y., Rubina M., and Frish K., Insect Sci Application 2, 157–161, 1981.
Calisher C.H., Carabatsos N., Dalrymple J.M., Shope R.E., Portafield J.S., Westaway E.G., and Brandt W.E., J Gen Virol 70, 37–43, 1989.
Chambers T.J., Hahn C.S., Goller R., and Rice C.M., Annu Rev Microbiol 44, 649–688, 1990.
Fulop L., Barett A.D.T., Phillpotts R., Martin K., Leslie D., and Titball R.W., J Virol Method 44, 179–188, 1993.
Goldblum M., Sterk V.V., and Paderski B., Am J Hyg 59, 89–103, 1954.
Ianconescu M., Avian Dis 20, 135–138, 1976.
Ianconescu M., in Hitchner S.B., Domermuth C.H., Purchase H.G. and Williams J.E. (eds), Isolation and Identification of Avian Pathogens. Am Assoc. Avian Pathol, Kennett Square, PA, USA, 1980, pp. 83–84.
Ianconescu M, Aharonovich A., Samberg Y., Merdinger M., and Hornstein K., Refuah Veterinaria 29, 110–117, 1972.
Ianconescu M., Aharonovich A., Samberg Y., Hornstein K., and Merdinger, M., Avian Pathol 2, 251–262, 1973.
Ianconescu M., Hornstein K., Samberg Y., Aharonovich A., and Merdinger M., Avian Pathol 4, 119–131, 1975.
Ianconescu M., in Calnek B.W., Barnes H.J., Beard C.W., Reid W.M., and Yoder H.W. Jr (eds), Diseases of Poultry. Ames, lowa, Iowa State University Press. 1991, pp. 674–679.
Komorov A. and Kalmar E., Vet Rec 72, 257–261, 1960.
Malkinson M., in McFerran J.B. and McNulty M.S. (eds), Virus Infection of Birds. Elsevier Science Publishers, Amsterdam, London, New York, Tokyo, 1993, pp. 243–245.
Pearson W.R. and Lipman D.J., Proc Natl Acad Sci 85, 2444–2448, 1988.
Pierre V., Drouet M.T., and Deubel V., Res Virol 145, 93–104, 1994.
Poidinger M., Hall R.A., and Mackennzie J.S., Virology 218, 417–421, 1996.
Portefield J.S., Vet Rec 73, 392–394, 1961.
Rice C.M., in Fields B.N., Knipe D.M., Chanock R.M., Monath T.P., Howley P.M., Melnick J.L., and Roizman B. (eds), Virology, III Edition Vol. 1. Lippincott-Raven, Philadelphia, New York, 1996, pp. 935–936.
Samberg Y., Ianconescu M., and Hornstein K., Refu Vet 29, 103–110, 1972.
Simpson D.I.H., in McFerran J.B. and McNulty M.S. (eds) Virus Infection of Birds. Elsevier Science Publishers, Amsterdam, London, New York, Tokyo, 1993, pp. 231–237.
Tanaka M., J Virol Method 41, 311–322, 1993.
Wengler G., Bradley D.W., Coliett M.S., Heintz F.X., Schlesinger R.W., and Strauss J.H., in Murphy F.A., Fauquet C.M., Bishop D.H.L., Ghabrial S.A., Jarvis A.W., Martelli G.P., Mayo M.A., and Summers M.D. (eds), Virus Taxonomy, Sixth Report of the International Committee on Taxonomy of Viruses. Springer Verlag, Wien/New York, 1995, pp. 415–427.
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Davidson, I., Lachmi, BE. & Weisman, Y. Development of RT-PCR for Turkey Meningoencephalitis Virus and Partial Sequence Analysis of the NS5 Gene. Virus Genes 16, 211–224 (1998). https://doi.org/10.1023/A:1007924224864
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DOI: https://doi.org/10.1023/A:1007924224864