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Herpesvirus of Turkeys Homologue of HSV VP16 Is Structurally Related to Varicella Zoster Virus Trans-Inducing Protein Encoded by ORF 10

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Abstract

Expression of the immediate-early genes of α-herpesviruses is stimulated by a family of trans-inducing factors represented by VP16 of HSV-1 and ORF10 gene product of VZV. We have identified and determined the nucleotide sequence of the UL48 gene encoding the herpesvirus of turkeys (HVT) homologue of HSV VP16. The gene maps to the BamHI-J fragment and appears to be expressed in a form of bicistronic transcript together with UL49. The deduced amino acid sequence of the protein encoded by HVT UL48 gene shows 55% identity with MDV UL48 gene product. Like the majority of related proteins in other α-herpesviruses, the protein encoded by HVT UL48 gene lacks the acidic C-terminal tail, known to possess the transactivation capacity of HSV VP16. Hydrophobic cluster analysis has revealed that its predicted domain composition is closely related to the transactivator protein encoded by ORF10 of VZV. However, the putative amino-terminal activation domain of the HVT homologue of HSV VP16 does not contain a typical horseshoe-like hydrophobic cluster found in other α-herpesvirus homologues, suggesting either that it acts as a transactivator via a different activation domain or that its transactivation potential is diminished.

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Authors and Affiliations

  1. Institute of Virology, Slovak Academy of Sciences, Dúbravská cesta 9, 842 46, Bratislava, Slovak Republic

    Juraj Kopáček, Vladimír Zelník, Dana Koptidesová, Silvia Pastoreková & Jaromír Pastorek

  2. Faculty of Agricultural Sciences, Centre of Biophysics and Molecular Numerics, B-5030, Gembloux, Belgium

    Robert Brasseur

  3. MEVAK a.s., Nitra, Slovak Republic

    Ol'ga Rejholcová

Authors
  1. Juraj Kopáček
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  2. Vladimír Zelník
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  3. Dana Koptidesová
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  4. Silvia Pastoreková
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  5. Jaromír Pastorek
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  6. Robert Brasseur
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  7. Ol'ga Rejholcová
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Kopáček, J., Zelník, V., Koptidesová, D. et al. Herpesvirus of Turkeys Homologue of HSV VP16 Is Structurally Related to Varicella Zoster Virus Trans-Inducing Protein Encoded by ORF 10. Virus Genes 15, 45–52 (1997). https://doi.org/10.1023/A:1007911115049

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  • DOI: https://doi.org/10.1023/A:1007911115049

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