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A comparison of sex steroid binding protein (SBP) in four species of teleost fish

Fish Physiology and Biochemistry volume 23, pages 245–256 (2000)Cite this article

Abstract

A sex steroid binding protein (SBP) binding E2 with high affinity has been detected in a pleuronectid (greenback flounder Rhombosolea tapirina), two sparids (black bream Acanthopagrus butcheri and snapper Pagrus auratus), and its presence has been confirmed in a salmonid (rainbow trout Oncorhynchus mykiss). SBP binding characteristics were measured using a hot saturation assay for trout, bream and snapper, and a cold saturation assay for flounder. Bound and unbound steroid were separated by incubation with dextran-coated charcoal (DCC). Affinity for E2 was highest in trout (kD=0.44 nM), followed by bream (kD=3.39 nM) and snapper (kD=10.7 nM). The lowest affinity was found in flounder (kD=84.7 nM). Binding capacity, however, was greatest in flounder (Bmax=164 nM), followed by trout (Bmax=92 nM), and then bream and snapper (Bmax=50 and 39 nM, respectively). Binding of E2 to SBP had a very rapid rate of association, and most dissociation occurred within 5 min. To confirm that the plasma protein measured here was SBP, the relative binding affinities of SBP for a range of steroids were measured. In trout, bream and snapper, SBP bound E2 with the highest affinity, followed by T. In contrast, the relative affinity of T for flounder SBP was more than twice that of E2. The rank orders of affinity of binding indicate the importance of an unhindered 17β-hydroxyl group, and a 3-hydroxyl or 3-ketone group for high affinity binding to SBP. These requirements for high affinity binding are present in most animals possessing SBP and indicate conservation of the SBP molecule through evolution.

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Authors and Affiliations

  1. School of Aquaculture, Locked Bag 1-370, Australia

    A.C. Hobby & N.W. Pankhurst

  2. School of Biomedical Science, University of Tasmania, Launceston, Locked Bag 1-320, Tasmania, 7250, Australia

    D.P. Geraghty

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  1. A.C. Hobby
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  2. N.W. Pankhurst
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  3. D.P. Geraghty
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Hobby, A., Pankhurst, N. & Geraghty, D. A comparison of sex steroid binding protein (SBP) in four species of teleost fish. Fish Physiology and Biochemistry 23, 245–256 (2000). https://doi.org/10.1023/A:1007869713348

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  • DOI: https://doi.org/10.1023/A:1007869713348

  • estradiol
  • salmonid
  • pleuronectid
  • sparid
  • teleost reproduction
  • binding protein

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