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Gonadotropin-releasing hormone (GnRH) neuronal systems in the pejerrey, Odontesthes bonariensis (Atheriniformes)

Fish Physiology and Biochemistry volume 23, pages 215–223 (2000)Cite this article

Abstract

The brain of the pejerrey (Odontesthes bonariensis) has recently been shown to contain three forms of gonadotropin-releasing hormone (GnRH): salmon GnRH (sGnRH), chicken GnRH-II (cGnRH-II) and pejerrey GnRH (pjGnRH), nevertheless neuroanatomical studies on the distribution of these peptides are lacking. In this study we investigated the distribution of immunoreactive GnRH in the brain of adult pejerrey. Four different policlonal antisera and a monoclonal antibody against different GnRH variants were applied on cryosections and visualized using the ABC method. Three antisera (PBL#49, sGnRH#2 and cII741) revealed three different immunoreactive areas: the terminal nerve ganglion (at the junction between the olfactory bulbs and the anterior telencephalon), the preoptic area just anterior to the hypothalamus and the midbrain tegmentum. Fibers immunoreactive to GnRH were detected in different brain areas: the olfactory bulbs, the ventral thelencephalon, the hypothalamus, the mesencephalic area and an important innervation entering into the pituitary gland. Two other antibodies (LRH13 and s1668) labeled the two nuclei corresponding to the forebrain but not the midbrain tegmentum. As both antibodies have low crossreactivity to cGnRH-II, the data suggest that this group of cells express cGnRH-II. In summary, three different areas with immunoreactivity to GnRH were detected in the pejerrey brain. The distribution of sGnRH, pjGnRH and cGnRH-II expressing neurons, is discussed.

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Authors and Affiliations

  1. Instituto de Neurociencia, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428, Buenos Aires, Argentina

    A.V. Stefano & J.M. Affanni

  2. Facultad de Medicina, Universidad de Morón, Machado 914, Morón, 1708, Buenos Aires, Argentina

    H.J. Aldana-Marcos

  3. Instituto de Investigaciones Biomédicas, Fundación Pablo Cassará, Saladillo 2452, C1440FFX 1440, Buenos Aires, Argentina

    G.M. Somoza

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  1. A.V. Stefano
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  2. H.J. Aldana-Marcos
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  3. J.M. Affanni
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  4. G.M. Somoza
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Stefano, A., Aldana-Marcos, H., Affanni, J. et al. Gonadotropin-releasing hormone (GnRH) neuronal systems in the pejerrey, Odontesthes bonariensis (Atheriniformes). Fish Physiology and Biochemistry 23, 215–223 (2000). https://doi.org/10.1023/A:1007841813926

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  • DOI: https://doi.org/10.1023/A:1007841813926

  • GnRH
  • immunohistochemistry
  • midbrain
  • pejerrey
  • preoptic area
  • terminal nerve

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