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Mechanisms of action of pituitary adenylate cyclase-activating polypeptide (PACAP) on growth hormone release from dispersed goldfish pituitary cells

Fish Physiology and Biochemistry volume 23, pages 201–214 (2000)Cite this article

Abstract

The mechanisms of pituitary adenylate cyclase activating polypeptide (PACAP) action on goldfish growth hormone (GH) release were investigated by examining GH release responses from dispersed goldfish pituitary cells to a synthetic mammalian (m)PACAP38 peptide. It was established that GH release stimulated by 2-h exposure to mPACAP38 was concentration-dependent, attenuated by the PACAP receptor antagonist mPACAP6−38, and subject to neuroendocrine modulation by somatostatin. Maximal mPACAP38-stimulated GH release was not additive to the responses elicited by either the adenylate cyclase activator forskolin or the cyclic (c)AMP analog 8-bromo-cAMP. The GH responses to mPACAP38, forskolin and 8-bromo-cAMP, either alone or in combination, were abolished by H89, a protein kinase A (PKA) inhibitor. SQ22536, an adenylate cyclase inhibitor, attenuated forskolin- and mPACAP38-stimulated GH release. In contrast, mPACAP38-stimulated GH release were additive to the responses to two protein kinase C (PKC) activators and unaffected by two PKC inhibitors. These results suggest that the stimulatory action of PACAP on GH secretion is mediated through a cAMP- / PKA-dependent mechanism, whereas the involvement of PKC appears unlikely. The ability of mPACAP38 to further enhance maximal GnRH (PKC)-dependent GH release, but not dopamine D1 agonist (PKA)-dependent GH secretion, is consistent with this hypothesis. A possible involvement of Ca2+ in PACAP action is also suggested. Two inhibitors of voltage-sensitive Ca2+ channel reduced the GH responses to mPACAP38 in static incubation; conversely, mPACAP38 increased intracellular [Ca2+] in identified, single goldfish somatotropes.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada

    Noel R. Wirachowsky, Patrick Kwong, Warren K. Yunker, James D. Johnson & John P. Chang

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  1. Noel R. Wirachowsky
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  2. Patrick Kwong
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  3. Warren K. Yunker
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  4. James D. Johnson
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  5. John P. Chang
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Wirachowsky, N.R., Kwong, P., Yunker, W.K. et al. Mechanisms of action of pituitary adenylate cyclase-activating polypeptide (PACAP) on growth hormone release from dispersed goldfish pituitary cells. Fish Physiology and Biochemistry 23, 201–214 (2000). https://doi.org/10.1023/A:1007837708880

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  • DOI: https://doi.org/10.1023/A:1007837708880

  • signal transduction
  • cyclic AMP
  • protein kinase A
  • protein kinase C
  • [Ca2+]i
  • voltage-sensitive Ca2+ channels

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