Abstract
The creation of enriched microsatellite libraries can ensure an abundant supply of microsatellite sequences at a considerably reduced cost. We report here the modification to an existing enrichment protocol and the optimisation of this technique for use on specific plant species, in this case, sugarcane. Using this optimised protocol, we achieved a 40% increase in the level of enrichment.
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Cregan PB, Akkaya MS, Bhagwat AA, Lavi U, Rongwen J (1994) Length polymorphisms of simple sequence repeat (SSR) DNA as molecular markers in plants, pp 47-56. In: P.M. Gresshoff (ed.), Plant Genome Analysis. CRC Press, Boca Raton, Florida.
Edwards KJ, Barker JHA, Daly A, Jones C, Karp A (1996) Microsatellite libraries enriched for several microsatellite sequences in plants. Biotechniques 20: 758–760.
Ma ZQ, Roder M, Sorrells ME (1996) Frequencies and sequence characteristics of di-, tri-, and tetra-nucleotide microsatellites in wheat. Genome 39: 123–130.
Saghai Maroof MA, Biyashev RB, Yang GP, Zhang Q, Allard RW (1994) Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations and population dynamics. Proc Natl Acad Sci USA 91: 5466–5470.
Weber JL (1990) Human DNA polymorphisms based on length variations in simple-sequence tandem repeats, pp. 159–181. In: KE Davis and SM Tilghman (eds.), Genome Analysis. Vol 1: Genetic and Physical Mapping. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
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Cordeiro, G.M., Maguire, T.L., Edwards, K.J. et al. Optimisation of a Microsatellite Enrichment Technique in Saccharum spp.. Plant Molecular Biology Reporter 17, 225–229 (1999). https://doi.org/10.1023/A:1007640231900
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DOI: https://doi.org/10.1023/A:1007640231900