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Optimisation of a Microsatellite Enrichment Technique in Saccharum spp.

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Abstract

The creation of enriched microsatellite libraries can ensure an abundant supply of microsatellite sequences at a considerably reduced cost. We report here the modification to an existing enrichment protocol and the optimisation of this technique for use on specific plant species, in this case, sugarcane. Using this optimised protocol, we achieved a 40% increase in the level of enrichment.

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Authors and Affiliations

  1. Centre for Plant Conservation Genetics, Southern Cross, University, PO Box 157, Lismore, NSW, 2480, Australia

    Giovanni M. Cordeiro, Tina L. Maguire & Robert J. Henry

  2. Centre for Coastal Management, Southern Cross University, PO Box 157, Lismore, NSW, 2480, Australia

    Tina L. Maguire

  3. IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol, BS18 9AF, UK

    Keith J. Edwards

Authors
  1. Giovanni M. Cordeiro
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  2. Tina L. Maguire
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  3. Keith J. Edwards
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  4. Robert J. Henry
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Cordeiro, G.M., Maguire, T.L., Edwards, K.J. et al. Optimisation of a Microsatellite Enrichment Technique in Saccharum spp.. Plant Molecular Biology Reporter 17, 225–229 (1999). https://doi.org/10.1023/A:1007640231900

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  • DOI: https://doi.org/10.1023/A:1007640231900

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