Abstract
Physical mapping of an arabidopsis DNA sequence of interest can easily be performed by PCR. This is done by using specific primers and pooled DNA templates isolated from publicly available YAC or BAC libraries. We present simple protocols for preparing pooled YAC DNAs and PCR-based detection of sequences in them by which several sequences can be mapped in a short time.
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References
Cooke R et al. (1996) Further progress towards a catalogue of all arabidopsis genes: analysis of a set of 5000 non-redundant ESTs. Plant J 9: 101–124.
Creusot et al. (1995) The CIC library: a large insert YAC library for genome mapping in Arabidopsis thaliana. Plant J 8: 763–770.
Frandsen G, Muller-Uri F, Nielsen M, Mundy J, Skriver K (1996) Novel plant Ca-2+-binding protein expressed in response to abscisic acid and osmotic stress. J Biol Chem 271: 343–348.
Philippsen P, Stotz A and Scherf C (1991) DNA of Saccharomyces cerevisiae. Meth Enzymol 194: 169–82.
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Naested, H., Jensen, A.B. & Mundy, J. Preparation of Pooled Arabidopsis YAC DNAs for PCR-Based Mapping. Plant Molecular Biology Reporter 17, 67–71 (1999). https://doi.org/10.1023/A:1007564130408
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DOI: https://doi.org/10.1023/A:1007564130408