Abstract
In previous studies we have shown that recombination across a 299-bp interspersed sequence accounts for the diversity of the mitochondrial genome in wild and cultivated soybeans. In this study, a computer-assisted survey of databases was performed using sequences of the repeat and its neighboring regions as query sequences. The sequences of soybean were found to be present as many short segments that include repeated sequences in the mitochondrial genomes of Arabidopsis and liverwort. Taken together with the results of a DNA gel-blot analysis, this suggests that the soybean sequences were found to have originated during land plant evolution and are present as small-interspersed segments in many taxa of land plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Altschul SF, Gish W, Miller W, Myers EW and Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403–410.
Andre C, Levy A and Walbot V (1992) Small repeated sequences and the structure of plant mitochondrial genomes. Trends Genet 8: 128–132.
Doyle JJ and Doyle JL (1987) A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11–15.
Kanazawa A, Tsutsumi N and Hirai A (1994) Reversible changes in the composition of the population of mtDNAs during dedifferentiation and regeneration in tobacco. Genetics 138: 865–870.
Kanazawa A, Tozuka A, Akimoto S, Abe J and Shimamoto Y (1998a) Phylogenetic relation-ships of the mitochondrial genomes in the genus Glycine subgenus Soja. Genes Genet Syst 73: 255–261.
Kanazawa A, Tozuka A, Kato S, Mikami T, Abe J and Shimamoto Y (1998b) Small in-terspersed sequences that serve as recombination sites at the cox2 and atp6 loci in the mitochondrial genome of soybean are widely distributed in higher plants. Curr Genet 33: 188–198.
Kato S, Kanazawa A, Mikami T and Shimamoto Y (1998) Evolutionary changes in the structures of the cox2 and atp6 loci in the mitochondrial genome of soybean involving recombination across small interspersed sequences. Curr Genet 34: 303–312.
Newton KJ (1988) Plant mitochondrial genomes: organization, expression and variation. Ann Rev Plant Physiol Plant Mol Biol 39: 503–532.
Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogura Y, Kohchi T and Ohyama K (1992) Gene organization deduced from the complete sequence of liverwort Marchantia polymorpha mitochondrial DNA. J Mol Biol 223: 1–7.
Shimamoto Y, Fukushi H, Abe J, Kanazawa A, Gai J, Gao Z and Xu D (1998) RFLPs of chloroplast and mitochondrial DNA in wild soybean, Glycine soja, growing in China. Genet Resour Crop Evol 45: 433–439.
Small ID, Suffolk R and Leaver CJ (1989) Evolution of plant mitochondrial genomes via substoichiometric intermediates. Cell 58: 69–76.
Tozuka A, Fukushi H, Hirata T, Ohara M, Kanazawa A, Mikami T, Abe J and Shimamoto Y (1997) Composite and clinal distribution of Glycine soja in Japan revealed by RFLP analysis of mitochondrial DNA. Theor Appl Genet 96: 170–176.
Unseld M, Marienfeld JR, Brandt P and Brennicke A (1997) The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366924 nucleotides. Nature Genet 15: 57–61.
Zeng D, Nielsen BL and Daniell H (1997) A 7.5-kbp region of the maize (T cytoplasm) mitochondrial genome contains a chloroplast-like trnI (CAT) pseudo gene and many short segments homologous to chloroplast and other known genes. Curr Genet 32: 125–131.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kanazawa, A., Shimamoto, Y. Soybean Recombination Sites are Present as Dispersed Segments in Arabidopsis and Liverwort Mitochondrial DNA. Plant Molecular Biology Reporter 17, 19–29 (1999). https://doi.org/10.1023/A:1007543717225
Issue Date:
DOI: https://doi.org/10.1023/A:1007543717225