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Effects of Protonophores on Membrane Electrical Characteristics in NG108-15 Cells

Neurochemical Research volume 25, pages 263–268 (2000)Cite this article

Abstract

Studies were conducted to determine the effects of bath application of the protonophores carbonyl cyanide m-chlorophenylhydrazone (CCCP) and carbonyl cyanide p-(trifluoromethoxy)-phenylhydrazone (FCCP) on membrane electrical characteristics of differentiated NG108-15 (neuroblastoma X glioma hybrid) cells. Membrane resting potential (Vm), input resistance (Rin) and electrically induced action potential generation were measured using intracellular micro-electrode techniques. Both compounds produced concentration-dependent depolarization rather than the hyperpolarization commonly found with other central mammalian neurons. CCCP and FCCP also reduced Rin and disrupted the generation of action potentials in a concentration-dependent manner. The contribution of the observed alterations to the in vivo toxicity of these compounds remains to be established.

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  1. Neurotoxicology Branch, Pharmacology Division, US Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, MD, 21010

    Jeffrey A. Doebler

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Doebler, J.A. Effects of Protonophores on Membrane Electrical Characteristics in NG108-15 Cells. Neurochem Res 25, 263–268 (2000). https://doi.org/10.1023/A:1007531822068

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  • DOI: https://doi.org/10.1023/A:1007531822068

  • Action potential
  • CCCP
  • FCCP
  • In vitro model
  • membrane potential
  • membrane resistance
  • NG108-15 (neuroblastoma X glioma) hybrid cells