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New Benzodiazepines Alter Acetylcholinesterase and ATPDase Activities

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Abstract

This study examines the effect of new 1,5 benzodiazepines on acetylcholinesterase (AChE) and ATPDase (apyrase) activities from cerebral cortex of adult rats. Simultaneously, the effects of the classical 1,4-benzodiazepine on these enzymes were also studied for comparative purpose. The compounds 2-trichloromethyl-4-phenyl-3H-1,5-benzodiazepin and 2-trichloromethyl-4-(p-methyl-phenyl)-3H-1,5-benzodiazepin significantly inhibited acetylcholinesterase activity (p < 0.01) when tested in the range of 0.18–0.35 mM. The inhibition caused by these two new benzodiazepines was noncompetitive in nature. Similarly, at concentrations ranging from 0.063 to 0.25 mM, the 1,5 benzodiazepines inhibited ATP and ADP hydrolysis by synaptosomes from cerebral cortex (p < 0.01). However, the inhibition of nucleotide hydrolysis was uncompetitive in nature. Our results suggest that, although diazepam and the new benzodiazepines have chemical differences, they both presented an inhibitory effect on acetylcholinesterase and ATPDase activities.

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Authors and Affiliations

  1. Departamento de Química, Centro de Ciências Naturais e Exatas, Brazil

    Maria R. C. Schetinger

  2. Departamento de Ciências Biológicas, Centro de Educação, Ciências Biológicas e Artes, Universidade da Região da Campanha, 97300-000, São Gabriel, RS, Brasil

    Neuza M. Porto

  3. Departamento de Análises Clinicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brasil

    Maria B. Moretto

  4. Departamento de Química, Centro de Ciências Naturais e Exatas, Brazil

    Vera M. Morsch, João Batista T. da Rocha, Vânia Vieira, Franciele Moro, Roseli Tatto Neis, Sandra Bittencourt, Hélio G. Bonacorso & Nilo Zanatta

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Schetinger, M.R.C., Porto, N.M., Moretto, M.B. et al. New Benzodiazepines Alter Acetylcholinesterase and ATPDase Activities. Neurochem Res 25, 949–955 (2000). https://doi.org/10.1023/A:1007500424392

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