Abstract
Three amplification protocols were analyzed for error rate and generation of polymorphisms during RAPD analysis. Using a set of 240 primers, the protocols detected similar frequencies of polymorphisms in two inbred sugar beet lines. The error rate was investigated by including a 1:1 mixture of DNA from the two lines in all analyses. Similar error rates, approximately 18%, were detected by the three protocols. Thus, altered amplification conditions did not substantially affect the error rate during RAPD analysis. For each of the three possible pairs of protocols, a positive correlation was obtained for primer and number of polymorphisms. Thus, a set of highly polymorphic RAPD primers can be used effectively, without prior screening, to detect polymorphisms for each protocol.
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Hansen, M., Halldén, C. & Sãll, T. Error Rates and Polymorphism Frequencies for Three RAPD Protocols. Plant Molecular Biology Reporter 16, 139 (1998). https://doi.org/10.1023/A:1007485919390
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DOI: https://doi.org/10.1023/A:1007485919390