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Detection of anti-hepatitis C-virus antibodies and hepatitis C-virus RNA in Lebanese hemodialysis patients

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Abstract

Hemodialysis patients are at high risk of developing HCV infection. Reports from various countries have shown a prevalence of 12–29% among this group. The present study aimed at assessing the utility of HCV antibodies and HCV-RNA detection in the diagnosis of HCV in Lebanese hemodialysis patients. One hundred and eight hemodialysis patients from various hospitals in Lebanon were assayed for the presence of anti-HCV antibodies by ELISA and LIA, and for the presence of HCV-RNA by RT-PCR of the 5′ Non-coding region (5′ NCR). Specificity of the amplicons was confirmed by Southern hybridization. Seventeen out of 108 patients were reactive in ELISA and positive in the Line Immunoassay (LIA). Eleven out of the 17 were positive by RT-PCR. Three out of 29 patients non-reactive in ELISA were positive by RT-PCR. Our results indicate that hemodialysis patients in this study may be grouped into 4 categories. These include (1) patients with viremia and noimmune response, (2) patients with no viremia and with an immune response, (3) patients with both viremia and immune response and (4) patients with no viremia and no immune response. The first 3 categories may reflect the different phases of HCV infection and imply that detection of both anti-HCV antibodies and HCV-RNA are needed for the establishment of adequate diagnosis. In addition, data collected from patients implicated in this study show that infection by HCV may be dialysis machine-related, rather than transfusion-related. However, cross-contamination unrelated to machines may also occurs.

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References

  1. Purcell RH. Hepatitis C-virus. In: Webster RG, Granoff A (eds), Encyclopedia of Virology, Vol. 2. San Diego: Academic Press Inc., 1994: 569–574.

    Google Scholar 

  2. Cordoba J, Camps J, Esteban JI. The clinical picture of acute and chronic hepatitis C. Curr Stud Hematol Blood Trans 1994; 61: 69–88.

    Google Scholar 

  3. Shapiro CN. Transmission of hepatitis viruses. Ann Intern Med 1994; 120: 82–84.

    Google Scholar 

  4. Schlipkoter U, Roggendorf M, Ernst G, Rasshofer R, Dienhardt F, Wiese A, Gladziwa U, Luz N. Hepatitis C-virus antibodies in hemodialysis patients. Lancet 1990; 335: 1409.

    Google Scholar 

  5. Moroni GA, Cori P, Marelli F, Delprefe M, Padovese P, Gallieni M, Anelli A, Brancaccio D. Indirect evidence for transfusion role in conditioning hepatitis C-virus prevalence among hemodialysis patients. Nephron 1991; 57: 371–372.

    Google Scholar 

  6. Araj GF, Kfoury-Baz EE, Barada KA, Nassif RE, Alami SY. Hepatitis C-virus; Prevalence in Lebanese blood donors and brief overview of the disease. J Med Lib 1995; 43: 11–16.

    Google Scholar 

  7. VanDoorn LJ. Molecular biology of the hepatitis C-virus (Review). J Med Virol 1994; 43: 345–346.

    Google Scholar 

  8. Cha TA, Kolberg J, Irvine B, Stempien M, Beall E, Yano M, Choo QL, Houghton M, Kuo G, Han JH, Urdea M. Use of signature nucleotide sequence of HCV for detection of viral RNA in human serum and plasma. J Clin Microbiol 1991; 29: 2528–2534.

    Google Scholar 

  9. Chan SW, McOmish F, Holmes EC, Dow B, Peutherer JF, Follett E, Yap PL, Simmonds P. Analysis of new hepatitis C-virus type and its phylogenetic relationship to existing variants. J Gen Virol 1992; 73: 1131–1141.

    Google Scholar 

  10. Popovic T, Bopp GA, Olsvik O, Kiehlbanch JA. Ribotyping in molecular epidemiology. In: Persing DH, Smith TF, Tenover FC, White TJ (eds), Diagnostic Molecular Microbiology, Principles and Applications. Washington, DC: American Society For Microbiology, 1993; 573–583.

    Google Scholar 

  11. Matar GM, Sharara HM, Abdelnour G, Abdelnoor A. Genotyping of hepatitis C-virus in Lebanese hemodialysis patients by RT-PCR-RF analysis of the 5′ non-coding region. J Clin Microbiol 1996; 34: 2623–2624.

    Google Scholar 

  12. Silini E, Bono F, Cerino A, Piazza V, Solcia E, Mondelli MU. Virological features of hepatitis C-virus infection in hemodialysis patients. J Clin Microbiol 1993; 31: 2931–2917.

    Google Scholar 

  13. Matinot-Peignoux M, Marcellin P, Xu LZ, Bernuou J, Erlinger S, Benhamou JP, Larzul D. Reactivity to C33c antigen as a marker of hepatitis C-virus multiplication. J Infect Dis 1992; 165: 595.

    Google Scholar 

  14. Houghton M, Wiener A, Jang H, Kuo J, Choo QL. Molecular biology of hepatitis C-viruses: Implications for diagnosis, development and control of viral diseases. Hepatology 1991; 14: 381–388.

    Google Scholar 

  15. Romeo R, Driss TF, Berthelot P, Nalpas B, Brechot C. Hepatitis C-virus RNA in serum of blood donors with or without elevated transaminase levels. Transfusion 1993; 33: 629–633.

    Google Scholar 

  16. De Medina M. Hepatitis C: Diagnostic assays. Semin Liver Dis 1995; 15: 33–40.

    Google Scholar 

  17. Schlipkoter U, Roggendorf M, Ernst G, Rasshofer R, Dienhardt F, Wiese A, Gladziwa U, Luz N. Hepatitis C-virus antibodies in hemodialysis patients. Lancet 1990; 335: 1409.

    Google Scholar 

  18. Farci P, Alter HJ, Govindarajan S, Wong DC, Engle R, Lesneiwski RR, Mushahwan IK, Desai SM, Miller RH, Ogata N, Purcell RH. Lack of protective immunity against re-infection with hepatitis C-virus. Science 1992; 258: 135–139.

    Google Scholar 

  19. Calabrese G, Vagelli G, Guaschino R, Gonella M. Transmission of HCV antibodies within the household of hemodialysis (Letter). Lancet 1991; 338: 1466.

    Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Faculty of Medicine, American University of Beirut, New York, NY, 10022, USA

    G.E. Abdelnour, G.M. Matar, H.M. Sharara & A.M. Abdelnoor

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  1. G.E. Abdelnour
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  2. G.M. Matar
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  3. H.M. Sharara
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  4. A.M. Abdelnoor
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Abdelnour, G., Matar, G., Sharara, H. et al. Detection of anti-hepatitis C-virus antibodies and hepatitis C-virus RNA in Lebanese hemodialysis patients. Eur J Epidemiol 13, 863–867 (1997). https://doi.org/10.1023/A:1007468322940

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