Abstract
We have modified the biolistic process to establish a transient assay for rapid analysis of transcription factors in arabidopsis. ATHB-1, an arabidopsis HD-Zip protein, was used as a transacting factor in particle bombardment experiments with arabidopsis leaves, and was found to transactivate a reporter gene in a binding site-specific manner. The experiments show the feasibility of using microprojectile-mediated gene transfer for rapid functional analysis of transcription factors in arabidopsis.
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Sessa, G., Borello, U., Morelli, G. et al. A Transient Assay for Rapid Functional Analysis of Transcription Factors in Arabidopsis. Plant Molecular Biology Reporter 16, 191 (1998). https://doi.org/10.1023/A:1007442607645
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DOI: https://doi.org/10.1023/A:1007442607645