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Microextraction of Nuclear Proteins from Single Maize Embryos

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Abstract

The analysis of DNA binding proteins can be difficult when only small quantities of tissue expressing the desired protein are available. We present a protocol for the preparation of nuclear extracts from as little as 100 mg of tissue. This protocol is well suited for extraction of DNA binding proteins from tissues that are difficult to obtain in large quantities such as maize embryos.

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Authors and Affiliations

  1. Departament de Genètica Molecular, Centre d'Investigació i Desenvolupament, C.S.I.C. Jordi Girona 18-26, 08034, Barcelona, Spain

    Peter K. Busk & Montserrat Pagès

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  1. Peter K. Busk
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  2. Montserrat Pagès
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Busk, P.K., Pagès, M. Microextraction of Nuclear Proteins from Single Maize Embryos. Plant Molecular Biology Reporter 15, 371–376 (1997). https://doi.org/10.1023/A:1007428802474

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