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Electrophoretic karyotyping and antifungal susceptibility patterns of Candida parapsilosis clinical isolates causing deep and superficial fungal infections

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Abstract

Forty-six isolates of Candida parapsilosis, each from a single patient, were collected from July 1993 through March 1999 at the University of Ancona Hospitals and Clinics. Twenty-eight strains were isolated from superficial lesioned sites, including skin, nails and other sources while 18 strains were isolated from blood. The isolates were typed by electrophoretickaryotyping (EK) and tested for their susceptibility to fluconazole (FLC),itraconazole (ITC), flucytosine (5-FC), and amphotericin B (AMB). Ourdata confirmed that EK is a useful technique for DNA typing of isolates ofCandida parapsilosis and showed that the source of isolation is notassociated with a given DNA type. Although strains belonging to this speciesof Candida are susceptible to the most common antifungals, including the triazoles, the degree of ITC susceptibility was dose dependent (MIC rangingfrom 0.25–0.5 μg/ml) for 98% of the isolates.

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References

  1. Nuyen MH, Peacock JE, Morris AJ, Tanner DC, Nguyen ML, Snydman DR, Wagener MM, Rinaldi MG, Yu VL. The changing face of candidemia: emergence of non Candida albicans species and antifungal resistance. Am J Med 1996; 100: 617-623.

    Article  Google Scholar 

  2. Cassone A, De Bernardis F, Pontieri E, Carruba G, Girmenia C, Martino P, Fernández-Rodríguez M, Quindós G, Pontón J. Biotype diversity of Candida parapsilosis and its relationship to the clinical source and experimental pathogenicity. J Infect Dis 1995; 171: 967-975.

    PubMed  CAS  Google Scholar 

  3. De Bernardis F, Lorenzini R, Verticchio R, Agatensi L, Cassone A. Isolation acid proteinase secretion, and experimental pathogenicity of Candida parapsilosis from outpatients with vaginitis. J Clin Microbiol 1989; 27: 2598-2603.

    PubMed  CAS  Google Scholar 

  4. Diekema DJ, Messer SA, Hollis RJ, Wenzel RP, Pfaller MA. An outbreak of Candida parapsilosis prosthetic valve endocarditis. Diagn Microbiol Infect Dis 1997; 29: 147-143.

    Article  PubMed  CAS  Google Scholar 

  5. Girmenia C, Martino P, De Bernardis F, Gentile G, Boccanera M, Monaco M, Antonucci G, Cassone A. Rising incidence of Candida parapsilosis fungemia in patients with hematologic malignancies: clinical aspects, predisposing factors, and differential pathogenicity of the causative strains. Clin Infect Dis 1996; 23: 506-514.

    PubMed  CAS  Google Scholar 

  6. Levin AS, Costa FS, Mussi NS, Basso M, Sinto SI, Machado C, Geiger DC, Villares MC, Schreiber AZ, Barone M, Branchini ML. Candida parapsilosis fungemia associated with implantable and semi-implantable central venous catheters and the hands of health care workers. Diagn Microbiol Infect Dis 1998; 30: 243-249.

    Article  PubMed  CAS  Google Scholar 

  7. Levy I, Rubin LG, Vasishtha S, Tucci V, Sood SK. Emergence of Candida parapsilosis as the predominant species causing candidemia in children. Olin Infect Dis 1998; 26: 1086-1088.

    CAS  Google Scholar 

  8. Waggoner-Fountain LA, Walker MW, Hollis RJ, Pfaller MA, Ferguson JE, Wenzel RP, Donowitz LG. Vertical and horizontal transmission of unique Candida species to premature newborns. Olin Infect Dis 1996; 22: 803-808.

    CAS  Google Scholar 

  9. Weems JJ Jr. Candida parapsilosis: epidemiology, pathogenicity, clinical manifestations, and antimicrobial susceptibility. Olin Infect Dis 1992; 14: 756-766.

    Google Scholar 

  10. Warren NG, Shadomy HJ: Yeasts of medical importance. In: Balows A, Hausler DJ Jr, Herrmann KL, Isenberg HD, Shadomy HJ, eds. Manual of clinical microbiology. American Society for Microbiology, Washington DC 1991; 617-629.

    Google Scholar 

  11. Barchiesi F, Hollis RJ, McGough DA, Scalise G, Rinaldi MG, Pfaller MA. DNA subtypes and fluconazole susceptibilities of Candida albicans isolates from the oral cavities of patients with AIDS. Olin Infect Dis 1995; 20: 634-640.

    CAS  Google Scholar 

  12. Sneath PHA. Numerical taxonomy. In: Sneath PHA, Sokal RR, eds. The principles and practice of numerical classification. Freeman WH, San Francisco 1973, 230-234.

    Google Scholar 

  13. National Committee for Clinical Laboratory Standards. Reference method for broth dilution antifungal susceptibility testing of yeasts. Approved standard M27-A. Wayne, PA: NCCLS, 1997.

    Google Scholar 

  14. Branchini ML, Pfaller MA, Rhine-Chalberg J, Frempong I, Isenberg HD. Genotypic variation and slime production among blood and catheter isolates of Candida parapsilosis. J Olin Microbiol 1994; 32: 452-456.

    CAS  Google Scholar 

  15. Carruba G, Pontieri E, De Bernard is F, Martino P, Cassone A: DNA fingerprinting and electrophoretic karyotype of environmental and clinical isolates of Candida parapsilosis. J Olin Microbiol 1991; 29: 916-922.

    CAS  Google Scholar 

  16. Pfaller MA, Messer SA, Hollis RJ. Variations in DNA subtype, antifungal susceptibility, and slime production among clinical isolates of Candida parapsilosis. Diagn Microbiol Infect Dis 1995; 21: 9-14.

    Article  PubMed  CAS  Google Scholar 

  17. Pontieri E, Gregori L, Gennarelli M, Ceddia T, Novelli G, Dallapiccola B, De Bernardis F, Carruba G. Correlation of SfiI macrorestriction endonuclease fingerprint analysis of Candida parapsilosis isolates with source of isolation. J Med Microbiol 1996; 45: 173-178.

    Article  PubMed  CAS  Google Scholar 

  18. Riederer K, Fozo P, Khatib R. Typing of Candida albicans and Candida parapsilosis species-related limitations of electrophoretic karyotyping and restriction endonuclease analysis of genomic DNA. Mycoses 1998; 41: 397-402.

    Article  PubMed  CAS  Google Scholar 

  19. Vazquez JA, Boikov D, Boikov SG, Dajani AS. Use of electrophoretic karyotyping in the evaluation of Candida infections in a neonatal intensive care unit. Infect Contr Hosp Epidemiol 1997; 18: 32-37.

    Article  CAS  Google Scholar 

  20. Hoeprich PD, Ingraham JL, Kleker E, Winship MJ. Development of resistance to 5-fluorocytosine in Candida parapsilosis during therapy. J Infect Dis 1974; 130: 112-118.

    PubMed  CAS  Google Scholar 

  21. Seidenfeld SM, Cooper BH, Smith JW, Luby JP, Mackowiak PA. Amphotericin B tolerance: a characteristic of Candida parapsilosis not shared by other Candida species. J Infect Dis 1983; 147: 116-119.

    PubMed  CAS  Google Scholar 

  22. Tumbarello M, Bevilacqua N, Federico G, Morace G, Cauda R, Tacconelli E. Fluconazole-resistant Candida parapsilosis fungemia in patients with AIDS. Olin Infect Dis 1996; 22: 179-180.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Istituto di Malattie Infettive e Medicina Pubblica, Universitá degli Studi di Ancona, Ancona, Italy

    F. Barchiesi, L. Falconi Di Francesco, D. Arzeni, F. Caselli, A. Giacometti & G. Scalise

  2. Clinica Dermatologica, Universitá degli Studi di Ancona, Ancona, Italy

    O. Simonetti, A. Cellini & A.M. Offidani

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  1. F. Barchiesi
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  2. L. Falconi Di Francesco
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  3. D. Arzeni
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  4. F. Caselli
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  5. O. Simonetti
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  6. A. Cellini
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  7. A. Giacometti
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  8. A.M. Offidani
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  9. G. Scalise
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Barchiesi, F., Di Francesco, L.F., Arzeni, D. et al. Electrophoretic karyotyping and antifungal susceptibility patterns of Candida parapsilosis clinical isolates causing deep and superficial fungal infections. Mycopathologia 149, 117–121 (2001). https://doi.org/10.1023/A:1007279213485

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