Abstract
Glycosaminoglycans (GAGs) have been identified histochemically and biochemically in urothelium. They have been suggested to have an anti-adherence effect at the bladder surface important in the urothelial defence against bacterial and carcinogenic insult. The aim of this study was to investigate urinary GAGs excretion in patients with interstitial cystitis.
Urinary GAG excretion was determined in patients with interstitial cystitis (n:34; 16 males, 18 females) and healthy subjects (n:34; 16 males, 18 females). Urinary GAG determinations were made by the dimethymethylene blue method. Student's t-test was used for statistical analysis of the results.
Urinary GAG excretion was found to be elevated significantly in patients with interstitial cystitis (14.45±2.02 mg/g Cr) as compared to healthy subjects (10.11±2.3 mg/g Cr) (p<0.01). There was no significant difference in urinary GAG excretion between males and females in either the healthy subjects or in the patients.
Determination of urinary GAG excretion may be an important non-invasive test in the investigation of patients with interstitial cystitis.
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References
Thorone, I. D., Resnick, M. I.: A methodology for the characterisation of urinary glycosaminoglycans. J. Urol., 13, 995 (1984).
Lilly, L. D., Parson, C. L.: Bladder surface glycosaminoglycan is a human epithelial permeability barrier. Surg. Gynec., 171, 493 (1990).
Hurst, R. E., Parson, C. L., Roy, J. B.: Urinary glycosaminoglycan excretion as a laboratory marker in the diagnosis of interstitial cystitis. J. Urol., 149, 31 (1993).
Gambaro, G., Cicerello, S., Mastrosimone, G., Lawagnim, T., Baggio, B.: High urinary excretion of glycosaminoglycans. A possible marker of glomerular involvement in diabetes. Metabolism, 38, 419 (1989).
Nesse, A., Garbossa, G., Romero, M. C., Bogado, C. E., Zanchetta, J. R.: Glycosaminoglycans in urolithiasis. Nephron, 62, 36 (1992).
Mitsuhaski, H., Tsukada, Y., Ono, K., Yano, S., Naruse, T.: Urine glycosaminoglycans and neparan sulphate excretions in adult patients with glomerular diseases. Clin. Nephrology, 39, 231 (1993).
Konukoğlu, D., Akçay, T., Erözenci, A.: The importance of urinary glycosaminoglycan as a marker for superficial bladder tumors. Cancer Biochem. Biophys., 15, 91 (1995).
Parsons, C. L., Schmidt, J. D., Pollen, J. J.: Successful treatment of interstitial cystitis with sodium pentosanpolysulphate. J. Urol., 130, 51 (1983).
Gillenwater, J. Y., Wein, A. J.: Summary of the national institute of arthritis, diabetes, digestive and kidney diseases workshop on interstitial cystitis, National Institute of Health. J. Urol., 140, 203 (1988).
Pain, G., Naia, S., Dall'Amico, R., Chandetti, L., Zachello, E. E., Catassi, C.: Simple spectrophotometric quantification of urinary excretion of glycosaminoglycan sulphates. Clin. Chem., 32, 2073 (1986).
Goldberg, J. M., Cotlier, E.: Specific isolation and analysis of mucopolysaccharides (Glycosaminoglycan) from human urine. Clin. Chem. Acta, 41, 19 (1984).
Parsons, C. L., Greenspan, C., Mulholland, S. G.: The primary antibacterial defense mechanism of the bladder. Invest Urol., 13, 72 (1975).
Baggio, B., Gambaro, G., Cicerello, S., Mastrosimone, G., Manzaro, A., Pagano, F.: Urinary excretion of glycosaminoglycans in urological disease. Clin. Biochem., 20, 449 (1987).
Fellstrom, B., Danielson, B. G., Ljunghall, S., Wirkstrom, B.: Combined enzymatic degradation with chondroitinase and Alcian Blue precipitation in determination of urinary chondroit in sulphates. Urol. Res., 12, 91 (1981).
Whitley, C. P., Rdmour, M. D., Draper, K. A., Dutton, C. M., Negila, J. P.: Diagnostic test for mucopolysaccharidoses. I. Direct method for quantifying excessive urinary glycosaminoglycan excretion. Clin. Chem., 35, 374 (1989).
Pennock, C. A.: A review and selection of simple laboratory methods used for the study of glycosaminoglycan excretion in the diagnosis of the mucopolysaccharidoses. J. Clin. Path., 29, 111 (1976).
Hesse, A., Wuzel, H., Vahlensieck, W.: The excretion of glycosaminoglycans in the urine of calcium-oxalate-stone patients and healthy persons. Urology, 41, 81 (1986).
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Akçay, T., Konukoğlu, D. Glycosaminoglycans Excretion in Interstitial Cystitis. Int Urol Nephrol 31, 431–435 (1999). https://doi.org/10.1023/A:1007198708404
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DOI: https://doi.org/10.1023/A:1007198708404